Probiotic P-acidilactici application in shrimp Litopenaeus stylirostris culture subject to vibriosis in New Caledonia

Type Article
Date 2008-03
Language English
Author(s) Castex Mathieu1, 3, Chim Liet1, Pham Dominique1, Lemaire Pierrette1, Wabete NellyORCID1, Nicolas Jean-Louis2, Schmidely P3, Mariojouls C3
Affiliation(s) 1 : IFREMER, Dept Aquaculture Nouvelle Caledonie, Noumea, New Caledonia.
2 : IFREMER, Lab Physiol Invertebres, DRV A, Ctr Brest, F-29280 Plouzane, France.
3 : AgroParisTech, F-75231 Paris, France.
Source Aquaculture (0044-8486) (Elsevier), 2008-03 , Vol. 275 , N. 1-4 , P. 182-193
DOI 10.1016/j.aquaculture.2008.01.011
WOS© Times Cited 66
Keyword(s) Vibriosis, Floating cages, Litopenaeus stylirostris, Pediococcus acidilactici, Probiotic
Abstract We studied the effects of a lactic acid bacterium, Pediococcus acidilactici (strain MA 18/5M, CNCM), as a dietary probiotic on growth performance and some nutritional and microbiological aspects of the shrimp Litopenaeus stylirostris. Experimental shrimp culture was carried out over 10 weeks, using floating cages of 14 m(2) each set in earthen ponds, in a New Caledonia farm affected by "Summer syndrome", a septicaemic vibriosis caused by Vibrio nigripulchritudo. The experiment design testing probiotic-coated pellets against control was replicated in two earthen ponds. High mortalities, characteristic of Summer syndrome, were observed during the first 2 weeks of the trial. The probiotic improved production in the treated cages from both ponds with increases in the survival rate (7% and 15% respectively) and final biomass (8% and 12% respectively). No differences were recorded among treatments on growth performances, but significant lower food conversion ratios were obtained with probiotic treatment. After 5 weeks of rearing, the Hepatosomatic Index and the adjusted dry weight of the digestive gland were significantly increased by 10% and by 9% respectively in shrimps treated with probiotic. In the meantime, the specific activities of alpha amylase and trypsin in the digestive gland showed a significant effect of the probiotic treatment with respective increases by 35% and 55%. The rise in total trypsin activity following morning feeding was also enhanced by the probiotic treatment (P < 0.001).

The highest concentration of probiotic (between 10(4)-10(5) CFU g(-1) of fresh gut) in the shrimp gut was obtained 2 h after feeding. The concentration remained high for 4 h after feeding before decreasing until the next meal. Total bacterial counts on Marine agar and TCBS in the gut were significantly lowered by the probiotic treatment over the 10 weeks of the experiment. Additionally, during the first 2 weeks, prevalence and load of V nigripulchritudo strains in haemolymph was lower in animals fed with the probiotic diet.

This study demonstrated, under pond conditions, that feeding shrimp with live terrestrial lactic acid bacteria can be an effective treatment for improving shrimp culture affected by vibriosis. (c) 2008 Elsevier B.V. All rights reserved.
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