FN Archimer Export Format PT J TI Flow cytometric determination of genome size in European seabass (Dicentrarchus labrax), gilthead seabream (Sparus aurata), thinlip mullet (Liza ramada), and European eel (Anguilla anguilla) BT AF PERUZZI, Stefano CHATAIN, Beatrice MENU, Bruno AS 1:1,2;2:1;3:1; FF 1:PDG-DOP-DCM-PM-APOI;2:PDG-DOP-DCM-PM-APOI;3:PDG-DOP-DCM-PM-APOI; C1 IFREMER, Lab Rech Piscicole Mediterranee Chemin Maguelone, F-34250 Palavas Les Flots, France. Univ Tromso, Norwegian Coll Fishery Sci, N-9037 Tromso, Norway. C2 IFREMER, FRANCE UNIV TROMSO, NORWAY SI PALAVAS SE PDG-DOP-DCM-PM-APOI IN WOS Ifremer jusqu'en 2018 copubli-europe IF 0.905 TC 16 UR https://archimer.ifremer.fr/doc/2005/publication-411.pdf LA English DT Article DE ;Anguilla anguilla;Liza ramada;Sparus aurata;Dicentrarchus labrax;Teleosts;DNA content AB The nuclear DNA content in Dicentrarchus labrax, Sparus aurata, Liza ramada, and Anguilla anguilla, was measured by flow cytometric analysis. Male human leukocytes were used as internal reference cells. Nucleated fish erythrocytes were stained simultaneously to human leukocytes with a staining buffer containing propidium iodide (PI). Nuclear DNA contents of target species were estimated in relation to an assigned value of 7.0 pg DNA for male human leukocytes. The DNA content/nucleus (+/- CI) was 1.55 +/- 0.02 pg for D. labrax, 1.90 +/- 0.03 pg for S. aurata, 1.57 +/- 0.02 pg for L. ramada, and 2.43 +/- 0.04 pg for A. anguilla. Intraspecific DNA content variations ranged from 0-9% and averaged around 4% in all taxa. The results did not evidence significant differences in genome size between males and females in either D. labrax or L. ramada. PY 2005 SO Aquatic Living Resources SN 0990-7440 PU EDP Sciences VL 18 IS 1 UT 000228214900008 BP 77 EP 81 DI 10.1051/alr:2005008 ID 411 ER EF