FN Archimer Export Format PT J TI In-situ microcosms, a tool for assessment of pesticide impacts on oyster spat (Crassostrea gigas) BT AF STACHOWSKI HABERKORN, S QUINIOU, Francoise NEDELEC, M ROBERT, Rene LIMON, G DE LA BROISE, D AS 1:1;2:2;3:;4:3;5:4;6:1; FF 1:;2:PDG-DOP-DCN-BE-LBEX;3:;4:PDG-DOP-DCB-PFOM-PI;5:;6:; C1 Univ Bretagne Occidentale, F-29334 Quimper, France. IFREMER, Ctr Brest, Dept Biogeochim & Ecotoxicol, F-29280 Plouzane, France. IFREMER, Lab Phys Invertebras Marins, F-29840 Landunvez, France. Technopole Brest Iroise, Lab Idhesa, F-29280 Plouzane, France. C2 UBO, FRANCE IFREMER, FRANCE IFREMER, FRANCE IDHESA, FRANCE SI BREST ARGENTON SE PDG-DOP-DCN-BE-LBEX PDG-DOP-DCB-PFOM-PI IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 2.355 TC 10 UR https://archimer.ifremer.fr/doc/2008/publication-4134.pdf LA English DT Article DE ;Epoxiconazole;Bentazon;Pesticide;Toxicity;Oyster;Microcosm AB Effects of the herbicide Basamais (bentazon) and the fungicide Opus (epoxiconazole) on oyster spat (Crassostrea gigas) were assessed using in-situ microcosms in a field experiment lasting 13 days. Six-week-old hatchery spat (mean size 1.1 mm), previously collected on PVC plates, was immersed in glass bottles filled with 200 mu m filtered seawater. Bottles were maintained underwater at 6 m depth and their water content changed every other day. Growth, measured as shell area index increase, was 126 +/- 4% in the control bottles. While no growth differences were observed between control and individual pesticide treatments at 10 mu g l(-1), oysters treated with a mix of 10 mu g l(-1) Opus and 10 mu g l(-1) Basamais showed a 50% growth reduction compared with the control (P < 0.0001), suggesting a synergistic effect of these contaminants. Laboratory controls in microcosms maintained in a water bath with filtered natural light, were not significantly different from in-situ microcosm controls in the field, for organic weight content or growth. This in-situ experiment in microcosms allowed us to conclude that: (1) oyster spat can achieve significant growth in bottles immersed in situ without supplementary food; (2) this microcosm system is reliable and easy to use for environmental toxicity tests with C. gigas spat; (3) such microcosm systems can also be run in a laboratory water bath instead of more technically difficult immersed field experiments; (4) the synergistic effect observed here, at a concentration simulating a peak agricultural runoff event, suggests that the impacts of pesticides could be a real threat for oysters in estuarine areas. PY 2008 PD MAY SO Ecotoxicology SN 0963-9292 PU Springer VL 17 IS 4 UT 000254402500003 BP 235 EP 245 DI 10.1007/s10646-007-0190-9 ID 4134 ER EF