FN Archimer Export Format
PT J
TI Hepatic phosphoenolpyruvate carboxykinase gene expression is not repressed by dietary carbohydrates in rainbow trout (Oncorhynchus mykiss)
BT 
AF PANSERAT, Stéphane
   PLAGNES JUAN, E
   BREQUE, Jeanine
   KAUSHIK, Sadasivam
AS 1:;2:;3:;4:;
FF 1:;2:;3:;4:;
C1 INRA, IFREMER, Lab Fish Nutr, F-64310 St Pee Sur Nivelle, France.
C2 INRA, FRANCE
SI ST PEE SUR NIVELLE
SE PDG-DRV-RA-ARN
IN WOS Ifremer jusqu'en 2018
IF 2.478
TC 72
UR https://archimer.ifremer.fr/doc/2001/publication-414.pdf
LA English
DT Article
DE ;Gluconeogenesis;Nutrition;Metabolism;Glucose;Oncorhynchus mykiss;Rainbow trout;Phosphoenolpyruvate carboxykinase
AB Phosphoenolpyruvate carboxykinase (PEPCK) is a rate-limiting enzyme in hepatic gluconeogenesis and therefore plays a central role in glucose homeostasis. The aim of this study was to analyse the nutritional regulation of PEPCK gene expression in rainbow trout (Oncorhynchus mykiss), which are known to use dietary carbohydrates poorly. A full-length hepatic PEPCK cDNA (2637 base pairs with one open reading frame putatively encoding a 635-residue protein) was cloned and found to be highly homologous to mammalian PEPCKs. The presence of a putative peptide signal specific to a mitochondrial-type PEPCK in the deduced amino acid sequence suggests that this PEPCK gene codes for a mitochondrial form, In gluconeogenic tissues such as liver, kidney and intestine, this PEPCK gene was expressed at high levels and, in the liver we found no regulation of PEPCK gene expression by dietary carbohydrates. These results suggest that the first step of the hepatic gluconeogenic pathway in rainbow trout is functional and highly active irrespective of the dietary carbohydrate supply.
PY 2001
SO Journal of Experimental Biology
SN 0022-0949
PU Company of Biologists
VL 204
IS 2
UT 000166717400018
BP 359
EP 365
ID 414
ER

EF