FN Archimer Export Format PT J TI Nutritional regulation and tissue specificity of gene expression for proteins involved in hepatic glucose metabolism in rainbow trout (Oncorhynchus mykiss) BT AF PANSERAT, Stéphane PLAGNES JUAN, E KAUSHIK, Sadasivam AS 1:;2:;3:; FF 1:;2:;3:; C1 INRA, IFREMER, Lab Fish Nutr, F-64310 St Pee Sur Nivelle, France. C2 INRA, FRANCE SI ST PEE SUR NIVELLE SE PDG-DRV-RA-ARN IN WOS Ifremer jusqu'en 2018 IF 2.478 TC 121 UR https://archimer.ifremer.fr/doc/2001/publication-415.pdf LA English DT Article DE ;Oncorhynchus mykiss;Trout;Bifunctional enzyme;Pyruvate kinase;Fructose bisphosphatase;Glucose transporter;Glucose metabolism;Liver metabolism AB Rainbow trout (Oncorhynchus mykiss) are known to use dietary carbohydrates poorly. One of the hypotheses to explain the poor utilisation of dietary glucose by these fish is a dysfunction in nutritional regulation of hepatic glucose metabolism. In this study, we obtained partial clones of rainbow trout cDNAs coding for a glucose transporter (Glut2), and for the enzymes 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (6PF-2K/F-2,6BPase), fructose-1,6-bisphosphatase (FBPase) and pyruvate kinase (PI(), Their deduced amino acid sequences were highly similar to those of mammals (up to 80% similarity), In a study of nutritional regulation, the Glut2 gene was highly expressed in the liver irrespective of the nutritional status of the trout, in agreement with the role of this transporter in the input (during refeeding) and output (during fasting) of glucose from the liver, Moreover, whereas PK and FBPase gene expression was high irrespective of the nutritional status, levels of hepatic 6PF-2K/F-2,6BPase mRNA were higher in fish fed with carbohydrates than in fish deprived of food. The high levels of hepatic PK, Glut2 and 6PF-2K/F-2,6BPase gene expression observed in this study suggest a high potential for tissue carbohydrate utilisation in rainbow trout. The persistence of a high level of FBPase gene expression suggests an absence of regulation of the gluconeogenic pathway by dietary carbohydrates. PY 2001 PD JUN SO Journal of Experimental Biology SN 0022-0949 PU Company of Biologists VL 204 IS 13 UT 000170021400014 BP 2351 EP 2360 ID 415 ER EF