FN Archimer Export Format PT J TI Pavlova lutheri: Production, preservation and use as food for Crassostrea gigas larvae BT AF PONIS, Emanuele PARISI, G CHINI ZITTELLI, G LAVISTA, F ROBERT, Rene TREDICI, M. R. AS 1:2;2:2;3:3;4:1;5:4;6:1; FF 1:;2:;3:;4:;5:PDG-DOP-DCB-PFOM-PI;6:; C1 Univ Florence, Dipartimento Biotecnol Agr, I-50144 Florence, Italy. Univ Florence, Dipartimento Sci Zootecn, I-50144 Florence, Italy. CNR, Ist Ecosistemi, I-50019 Florence, Italy. IFREMER, Lab Physiol Invertebres Marins, F-29840 Presquile Du Vivier, Landunvez, France. C2 UNIV FLORENCE, ITALY UNIV FLORENCE, ITALY CNR, ITALY IFREMER, FRANCE SI ARGENTON SE PDG-DOP-DCB-PFOM-PI IN WOS Ifremer jusqu'en 2018 copubli-europe IF 1.678 TC 26 UR https://archimer.ifremer.fr/doc/2008/publication-4194.pdf LA English DT Article DE ;Crassostrea gigas;Fatty acid composition;Mollusc nutrition;Photobioreactor;Pavlova lutheri;Preservation;Algal suspensions AB The haptophyte Pavlova lutheri was cultivated in a 4-l flat alveolar photobioreactor (FAP) in semi-continuous mode during 35 days, achieving a mean volumetric productivity of 0.4 g l-1 day-1. The microalgal culture was harvested from the photobioreactor at an average concentration of 3.5 g l-1 and subjected to different storage conditions in which the effects of temperature (1 °C, 4 °C and 25 °C), air supply and preservative (ascorbic or propionic acid) addition on the viability of the stored suspensions were investigated. Low temperatures (1 and 4 °C) and air-bubbling extended the shelf-life of the stored suspension, while no beneficial effects of ascorbic or propionic acid were found. The influence of temperature and air-bubbling on fatty acid content and profile was also evaluated over a 23-day period of storage. Air-bubbled suspensions exhibited, on the average, a 70% decrease of total fatty acid content (TFA) during storage. Non-bubbled suspensions showed a much lower decrease. The decrease mainly involved the saturated 14:0 and 16:0 and the monounsaturated 16:1n-7 and 18:1n-9. The polyunsaturated eicosapentaenoic acid (20:5n-3; EPA) and docosahexaenoic acid (22:6n-3; DHA) decreased significantly only at the end of the storage period. A feeding trial on Pacific oyster (Crassostrea gigas) larvae was carried out with fresh and preserved P. lutheri biomass kept under the best storage conditions previously defined (1 °C; darkness; air-bubbling). After 14 days of rearing no significant differences in growth or survival were detected when fresh or preserved P. lutheri was used in a monospecific diet or in association with the diatom Chaetoceros calcitrans forma pumilum. PY 2008 PD JUL SO Aquaculture SN 0044-8486 PU Elsevier VL 282 IS 1-4 UT 000260292000015 BP 97 EP 103 DI 10.1016/j.aquaculture.2008.06.010 ID 4194 ER EF