FN Archimer Export Format
PT J
TI Purification and partial identification of novel antimicrobial protein from marine bacterium Pseudoalteromonas species strain x 4 53
BT 
AF LONGEON, Arlette
   PEDUZZI, Jean
   BARTHELEMY, Michel
   CORRE, Sophie
   NICOLAS, Jean-Louis
   GUYOT, Museum National d'Histoire Naturelle
AS 1:;2:;3:;4:;5:;6:;
FF 1:;2:;3:;4:;5:PDG-DOP-DCB-PFOM-PI;6:;
C1 CNRS, UMR 5154, Natl Museum Nat Hist, Lab Chim & Biochim Substances Nat,USM 502, F-75005 Paris, France.
   Micromer Technopole Brest Iroise, F-29200 Brest, France.
   IFREMER, DRV, Lab Physiol Invertebres, F-29280 Plouzane, France.
C2 CNRS, FRANCE
   MICROMER TECHNOPOLE BREST IROISE, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-DOP-DCB-PFOM-PI
IN WOS Ifremer jusqu'en 2018
IF 1.237
TC 60
UR https://archimer.ifremer.fr/doc/2004/publication-426.pdf
LA English
DT Article
DE ;Probiotic bacteria;Pseudoalteromonas;Antimicrobial protein
AB A marine bacterium, X 153, was isolated from a pebble collected at St. Anne du Portzic (France). By 16S ribosomal DNA gene sequence analysis, X153 strain was identified as a Pseudoalteromonas sp. close to P. piscicida. The crude culture of X153 was highly active against human pathogenic strains involved in dermatologic diseases, and marine bacteria including various ichthyopathogenic Vibrio strains. The active substance occurred both in bacterial cells and in culture supernatant. An antimicrobial protein was purified to homogeneity by a 4-step procedure using size-exclusion and ion-exchange chromatography. The highly purified P-153 protein is anionic, and sodium dodecylsulfate polyacrylamide gel electrophoresis gives all apparent molecular mass of 87 kDa. The X153 bacterium protected bivalve larvae against mortality, following experimental challenges with ichthyopathogenic Vibrio. Pseudoalteromonas sp. X153 may be useful in aquaculture as a probiotic bacterium.
PY 2004
PD NOV
SO Marine Biotechnology
SN 1436-2228
PU Springer
VL 6
IS 6
UT 000228866100013
BP 633
EP 641
DI 10.1007/s10126-004-3009-1
ID 426
ER

EF