||Boutet Isabelle, Tanguy Aurelie, Moraga Dario
||Univ Bretagne Occidentale, Inst Univ Europeen Mer, CNRS,UMR 6539, Lab Sci Environm Marin LEMAR, F-29280 Plouzane, France.
IFREMER, CNRS, UMR Genome Populat Interact Adaptat 5171, Stn Mediterraneenne Environm Littoral, F-34200 Sete, France.
||Marine Biology (0025-3162) (Springer), 2004-12 , Vol. 146 , N. 1 , P. 53-64
|WOS© Times Cited
||Crassostrea gigas, Gene expression, Multiplex RTPCR, Pesticides, Hydrocarbons, cDNA sequences, Glutathione S transferases
||Hydrocarbon and pesticide pollution in coastal ecosystems can disturb marine bivalve metabolism. In this study, we characterised four full-length cDNA sequences encoding glutathione S-transferases (GSTs) in the Pacific oyster Crassostrea gigas. A BLAST X search showed that these four sequences encode GSTs from four different classes: GST pi, sigma, mu and omega. A phylogenetic analysis of GST was made to determine the position of oyster GST compared to invertebrate and vertebrate sequences. We developed a semi-quantitative, multiplex RT-PCR to follow the expression of these four GSTs in tissues of oysters exposed to hydrocarbons and two pesticide treatments ( glyphosate and a mixture composed of atrazine, diuron and isoproturon) under experimental conditions. Our results showed strong differential expression of these four GSTs that was both tissue specific as well as time and treatment dependent. We observed that expression levels were higher in digestive gland than in gill tissues in pesticide-exposed oysters. Furthermore, omega and mu class GST mRNA expression in the digestive gland might be useful as a possible marker of hydrocarbon exposure, while pi and sigma class GST mRNA expression in the digestive gland may be similarly useful as a marker of pesticide exposure in monitoring programmes.