FN Archimer Export Format
PT J
TI Changes of gill and hemocyte-related bio-indicators during long term maintenance of the vent mussel Bathymodiolus azoricus held in aquaria at atmospheric pressure
BT 
AF BETTENCOURT, R
   DANDO, P
   ROSA, D
   RIOU, V
   COLACO, A
   SARRAZIN, Jozee
   SARRADIN, Pierre-Marie
   SANTOS, R
AS 1:1;2:2;3:1;4:1;5:1;6:3;7:3;8:1;
FF 1:;2:;3:;4:;5:;6:PDG-DOP-DCB-EEP-LEP;7:PDG-DOP-DCB-EEP-LEP;8:;
C1 Univ Azores, Genet & Mol Biol Lab, IMAR Dept Oceanog & Fisheries, P-9901862 Horta, Portugal.
   Marine Biol Assoc United Kingdom Lab, Plymouth PL1 2PB, Devon, England.
   Ifremer, Ctr Brest, Dept Etud Ecosyst Profonds, Lab Environm Profond, F-29280 Plouzane, France.
C2 UNIV AZORES, PORTUGAL
   MBA, UK
   IFREMER, FRANCE
SI BREST
SE PDG-DOP-DCB-EEP-LEP
IN WOS Ifremer jusqu'en 2018
   copubli-europe
IF 1.709
TC 17
UR https://archimer.ifremer.fr/doc/2008/publication-4357.pdf
LA English
DT Article
CR MOMARETO
BO Unknown
DE ;Vent;Hydrothermal vent;Bathymodiolus azoricus;Aquarium acclimatization;Phagocytosis;Alcian blue Periodic Acid Schiff staining;Mucopolysaccharide
AB The deep-sea hydrothermal vent mussel Bathymodiolus azoricus has been the subject of several studies aimed at understanding the physiological adaptations that vent animals have developed in order to cope with the particular physical and chemical conditions of hydrothermal environments. In spite of reports describing successful procedures to maintain vent mussels under laboratory conditions at atmospheric pressure, few studies have described the mussel's physiological state after a long period in aquaria. In the present study, we investigate changes in mucocytes and hemocytes in B. azoricus over the course of several months after deep-sea retrieval. The visualization of granules of mucopolysaccharide or glycoprotein was made possible through their inherent auto-fluorescent property and the Alcian blue-Periodic Acid Schiff staining method. The density and distribution of droplets of mucus-like granules was observed at the ventral end of lamellae during acclimatization period. The mucus-like granules were greatly reduced after 3 months and nearly absent after 6 months of aquarium conditions. Additionally, we examined the depletion of endosymbiont bacteria from gill tissues, which typically occurs within a few weeks in sea water under laboratory conditions. The physiological state of B. azoricus after 6 months of acclimatization was also examined by means of phagocytosis assays using hemocytes. Hemocytes from mussels held in aquaria up to 6 months were still capable of phagocytosis but to a lesser extent when Compared to the number of ingested yeast particles per phagocytic hemocytes from freshly collected vent mussels. We suggest that the changes in gill mucopolysaccharides and hemocyte glycoproteins, the endosymbiont abundance in gill tissues and phagocytosis are useful health criteria to assess long term maintenance of B. azoricus in aquaria. Furthermore, the laboratory set up to which vent mussels were acclimatized is an applicable system to study physiological reactions such as hemocyte immunocompetence even in the absence of the high hydrostatic pressure found at deep-sea vent sites.
PY 2008
PD MAY
SO Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
SN 1095-6433
PU Elsevier
VL 150
IS 1
UT 000256499700001
BP 1
EP 7
DI 10.1016/j.cbpa.2008.02.020
ID 4357
ER

EF