FN Archimer Export Format PT J TI Microsatellite analysis of 6-hour-old embryos reveals no preferential intraspecific fertilization between cupped oysters Crassostrea gigas and Crassostrea angulata BT AF HUVET, Arnaud BALABAUD, K BIERNE, Nicolas BOUDRY, Pierre AS 1:;2:;3:;4:; FF 1:PDG-DRV-RA-PI;2:;3:PDG-DRV-RA-DCOP-AT;4:PDG-DRV-RA-LGP; C1 IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France. IFREMER, CNRS, UMR 5000, Lab Genome Populat Interact, F-34200 Sete, France. C2 IFREMER, FRANCE IFREMER, FRANCE SI BREST TAHITI LA TREMBLADE SE PDG-DRV-RA-PI PDG-DRV-RA-DCOP-AT PDG-DRV-RA-LGP IN WOS Ifremer jusqu'en 2018 IF 1.408 TC 30 UR https://archimer.ifremer.fr/doc/2001/publication-436.pdf LA English DT Article DE ;Paternal contribution;Microsatellites;In vitro crossings;Hybridization;Crassostrea angulata;Crassostrea gigas AB Experimental examination of reproductive isolation is the first step in understanding li,hybridization processes. Here, we studied preferential fertilization between 2 cupped oyster taxa, Crassostrea angulata and Crassostrea gigas, as a potential prezygotic reproductive isolation. Early examination of sperm competition is now possible by molecular analysis of oyster embryos. This avoids the confounding effect of differential mortality during the larval stage. Six hundred embryos were sampled from 2 crosses. Three microsatellite loci were enough to determine without ambiguity the taxa of contributing sires of embryos. No evidence of preferential fertilization between gametes from the same taxa was shown. A significantly higher contribution of the C. gigas males was revealed with the C. angulata females, but not with the C. gigas females, which might suggest early heterosis or interaction differences between gametes. In the light of these results, natural hybridization between both taxa call be expected in cases of their geographical coexistence, as in the Southern European populations in which both taxa. are in contact as a result of aquaculture development. PY 2001 PD SEP SO Marine Biotechnology SN 1436-2228 PU Springer VL 3 IS 5 UT 000172433600005 BP 448 EP 453 DI 10.1007/s10126-001-0017-2 ID 436 ER EF