Shell repair process in the green ormer Haliotis tuberculata: A histological and microstructural study

Type Article
Date 2008-06
Language English
Author(s) Fleury C2, Marin F1, Marie B1, Luquet G1, Thomas J1, Josse C3, Serpentini A2, Lebel J2
Affiliation(s) 1 : Univ Bourgogne, CNRS, UMR 5561, Lab Biogeosci, F-21000 Dijon, France.
2 : Univ Caen Basse Normandie, IFREMER, UMR 100, IFR 146,ICORE,Lab Physiol & Ecophysiol Mollusques, F-14032 Caen, France.
3 : Univ Bourgogne, Lab React Solides, F-21078 Dijon, France.
Source Tissue and Cell (0040-8166) (Elsevier), 2008-06 , Vol. 40 , N. 3 , P. 207-218
DOI 10.1016/j.tice.2007.12.002
WOS© Times Cited 41
Keyword(s) Microstructure, Mantle epithelium, Biomineralization, Calcium carbonate, Shell repair, Gastropod
Abstract In the present paper, juvenile and adult shells of the green ormer Haliotis tuberculata ('Oreille de Saint-Pierre') were perforated in a zone close to the shell edge and the shell repair process was followed at two levels: (1) by observing the histology of the calcifying mantle in the repair zone and (2) by analyzing with SEM the microstructure of the shell repair zone. Histological data clearly show the presence of calcium carbonate granules into the connective tissues, but not in the epithelial cells. This suggests that calcium carbonate granules are synthesized by sub-epithelial cells and actively transported through the epithelium to the repair zone, via a process which may be similar to that described by Mount et al. [Mount, A.S., Wheeler, A.P., Paradkar, R.P., Snider, D., 2004. Hemocyte-mediated shell mineralization in the eastern oyster. Science 304, 297-300]. Furthermore, SEM observations show that the repair zone exhibits different stratified microstructures (spherulitic, thin prismatic, blocklike, sub-nacreous, nacreous, foliated-like), some of which are not continuous (i.e. lenticular) along the repair zone. This suggests a complex secreting regime of the calcifying mantle and an elaborate geometry of the epithelium involved in shell repair. (C) 2008 Elsevier Ltd. All rights reserved.
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