FN Archimer Export Format
PT J
TI Rapid and sensitive PCR detection of Vibrio penaeicida, the putative etiological agent of syndrome 93 in New Caledonia
BT 
AF SAULNIER, Denis
   AVARRE, Jean-Christophe
   LE MOULLAC, Gilles
   ANSQUER, Dominique
   LEVY, Peva
   VONAU, Vincent
AS 1:;2:;3:;4:;5:;6:;
FF 1:PDG-DRV-RA-LGP;2:PDG-DRV-RA-DRIM;3:PDG-DRV-RA-DCOP-AT;4:PDG-DRV-RA-NC;5:PDG-DRV-RA-DCOP-AT;6:PDG-DRV-RA-DCOP-AT;
C1 IFREMER, Ctr Oceanol Pacifique, Lab Aquaculture Trop, Taravao 98719, Tahiti, Fr Polynesia.
C2 IFREMER, FRANCE
SI LA TREMBLADE
   MONTPELLIER
   TAHITI
   SAINT VINCENT
SE PDG-DRV-RA-LGP
   PDG-DRV-RA-DRIM
   PDG-DRV-RA-DCOP-AT
   PDG-DRV-RA-NC
IN WOS Ifremer jusqu'en 2018
IF 1.556
TC 49
UR https://archimer.ifremer.fr/doc/2000/publication-4436.pdf
LA English
DT Article
DE ;small subunit rDNA gene;Penaeus (Litopenaeus) stylirostris;vibriosis;shrimp disease;PCR diagnosis;vibrio penaeicida;Syndrome 93
AB Experimental infections of Penaeus (Litopenaeus) stylirostris were performed with a Vibrio penaeicida strain (AM101) isolated in New Caledonia from Syndrome 93 diseased shrimp. Cumulative mortalities resulting from intramuscular injection or immersion of shrimp in bacterial suspensions demonstrated high virulence for this bacterial strain and suggested that V. penaeicida could be the etiological agent of Syndrome 93. The median lethal dose (LD50) for AM101 was 1.3 x 104 CFU (colony forming units) ml-1 by immersion and less than 5 CFU shrimp-1 by intramuscular challenge, with mortality outbreaks at 48 and 22 h after challenge, respectively. A polymerase chain reaction (PCR) detection assay using a primer set designed from the 16S ribosomal RNA gene of V. penaeicida was developed. It gave an expected amplicon of approximately 310 bp in ethidium bromide-stained agarose gels. The specificity of these primers was assessed with different Vibrio species. Furthermore, DNA extracted by the ChelexTM method could be used to detect fewer than 20 cultured Vibrio cells in seawater or shrimp hemolymph by this assay. It appears to be a reliable screening method for detecting V. penaeicida in shrimp and from the aquatic environment.
PY 2000
PD MAR
SO Diseases of aquatic organisms
SN 0177-5103
PU Inter-Research
VL 49
IS 2
UT 000086771500004
BP 109
EP 115
DI 10.3354/dao040109
ID 4436
ER

EF