TY - JOUR T1 - Activities of selected digestive enzymes during larval development of large yellow croaker (Pseudosciaena crocea) A1 - Ma,Hongming A1 - Cahu,Chantal A1 - Zambonino-Infante,Jose-Luis A1 - Yu,Hairui A1 - Duan,Qingyuan A1 - Le Gall,Marie-Madeleine A1 - Mai,Kangsen AD - Ocean Univ China, Minist Educ, Key Lab Mariculture, Qingdao 266003, Peoples R China. AD - INRA, IFREMER, Lab Fish Nutr, F-29280 Plouzane, France. UR - https://archimer.ifremer.fr/doc/00000/444/ DO - 10.1016/j.aquaculture.2004.11.032 KW - Trypsin KW - Pseudosciaena crocea KW - Fish larval development KW - Amylase KW - Aminopeptidase N KW - Alkaline phosphatase N2 - Large yellow croaker is an important marine fish species for aquaculture in China. Larval rearing of this species is still based on live prey feeding. The aim of this study was to describe the onset and development of the main digestive enzymes during larval development of this species, in order to obtain essential data for the formulation of a compound diet adapted to larvae. Enzymatic assays were conducted from day I post hatching to day 40 in larvae fed the following live prey sequence: rotifers, Artemia nauplii, copepods, and then frozen copepods. Assays were done on whole larvae until day 19, then on dissected larvae and on purified brush border membranes of intestine. Pancreatic enzymes, amylase, and trypsin, showed high activities from early stages, even before mouth opening. Mechanisms of pancreatic secretion were achieved from day 19. Enzymes of brush border membranes, alkaline phosphatase, and aminopeptidase N, increased abruptly between day 23 and 25, showing maturation of the intestinal digestive process. These data, together with data describing mouth enlargement during development, suggest that large yellow croaker larvae could be fed successfully with a compound diet from early stages, at least from day 23. Y1 - 2005/03 PB - Elsevier JF - Aquaculture SN - 0044-8486 VL - 245 IS - 1-4 SP - 239 EP - 248 ID - 444 ER -