FN Archimer Export Format PT J TI First report of a Mikrocytos-like parasite in European oysters Ostrea edulis from Canada after transport and quarantine in France BT AF GAGNE, N COCHENNEC, Nathalie STEPHENSON, M MCGLADDERY, S MEYER, G BOWER, S AS 1:1;2:3;3:1;4:2;5:4;6:4; FF 1:;2:PDG-DOP-DCOP-AQUAPOL-LBQP;3:;4:;5:;6:; C1 Fisheries & Oceans Canada, Gulf Fisheries Ctr, Moncton, NB E1C 9B6, Canada. Fisheries & Oceans Canada, Natl Capital Reg, Ottawa, ON K1A 0E6, Canada. IFREMER, Ctr Pacifique, F-98719 Taravao, Tahiti, Fr Polynesia. Fisheries & Oceans Canada, Pacific Biol Stn, Nanaimo, BC V9R 5K6, Canada. C2 MPO, CANADA MPO, CANADA IFREMER, FRANCE MPO, CANADA SI TAHITI SE PDG-DOP-DCOP-AQUAPOL-LBQP IN WOS Ifremer jusqu'en 2018 copubli-int-hors-europe IF 1.586 TC 19 UR https://archimer.ifremer.fr/doc/2008/publication-4475.pdf LA English DT Article DE ;Histology;PCR;In situ hybridization;Ostrea edulis;Mikrocytos AB As part of a disease resistance experiment, 112 apparently healthy European flat oysters Ostrea edulis L. were exported from Canada (Nova Scotia) into France to test their susceptibility to Bonamia ostreae infection. Twelve oysters died in transit and 17 others died within 2 wk of laboratory quarantine acclimation. All oysters were examined histologically, and the 17 that died during quarantine were assayed for microcells (Bonamia sp. and Mikrocytos mackini) using molecular techniques. A microcell parasite was detected in the connective tissue of 5 of the 112 oysters. Morphological appearance, tissue affinity and molecular characterization through PCR, in situ hybridization (ISH), fluorescence in situ hybridization (FISH) and sequencing revealed a protist related to M mackini. This is the first report of a parasite of the genus Mikrocytos in a species belonging to the genus, Ostrea from the Atlantic Ocean. PY 2008 PD JUL SO Diseases of aquatic organisms (depuis 2001) SN 0177-5103 PU Inter-Research VL 80 IS 1 UT 000257605200004 BP 27 EP 35 DI 10.3354/dao01922 ID 4475 ER EF