FN Archimer Export Format
PT J
TI Lysozyme gene expression and hemocyte behaviour in the Mediterranean mussel, Mytilus galloprovincialis, after injection of various bacteria or temperature stresses
BT 
AF LI, H
   PARISI, M
   TOUBIANA, Mylene
   CAMMARATA, M
   ROCH, Philippe
AS 1:1;2:1,2;3:1;4:2;5:1,3;
FF 1:;2:;3:;4:;5:;
C1 Univ Montpellier 2, CNRS, IFREMER, UMR Ecosyst Lagunaires 5119, F-34095 Montpellier 05, France.
   Univ Palermo, Marine Immunobiol Lab, I-90123 Palermo, Italy.
C2 IFREMER, FRANCE
   UNIV PALERMO, ITALY
   CNRS, FRANCE
SI MONTPELLIER
SE PDG-DRV-RA-DRIM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
IF 3.161
TC 76
UR https://archimer.ifremer.fr/doc/2008/publication-4479.pdf
LA English
DT Article
DE ;Molluscs;Mytilus;ISH;Q PCR;Gene expression;Lysozyme;Heat shock;Micrococcus lysodeikticus;Vibrio anguillarum;Vibrio splendidus
AB The aim of the present study was to evaluate the expression of the Mytilus galloprovincialis lysozyme gene in different in vivo stress situations, including injection of bacteria Vibrio splendidus LGP32, Vibrio anguillarum or Micrococcus lysodeikticus, as well as heat shock at 30 C and cold stress at 5 C. Injection of V. splendidus LGP32 resulted in: (i) a general down-regulation of lysozyme gene expression, as quantified by Q-PCR; (ii) reduction in the number of circulating hemocytes; (iii) decrease in the percentage of circulating hemocytes expressing lysozyme mRNA which was now restricted to only small cells, as observed by ISH; and (iv) accumulation of hemocytes expressing lysozyme in the muscle sinus where injection took place. Injection of V. anguillarum or M. lysodeikticus induced significant up-regulation of lysozyme gene expression, but only 2-3 days post-injection, with no change in the total hemocyte counts but an increased percentage of hemocytes expressing lysozyme mRNA. Neither the control injection of PBS-NaCl nor temperature stress modified the lysozyme expression pattern. Consequently, the hemocyte population appears to be capable of discriminating between stress factors, and even between 2 Vibrio species. (C) 2008 Elsevier Ltd. All rights reserved.
PY 2008
PD JUN
SO Fish & Shellfish Immunology
SN 1050-4648
PU Elsevier
VL 25
IS 1-2
UT 000257637600016
BP 143
EP 152
DI 10.1016/j.fsi.2008.04.001
ID 4479
ER

EF