FN Archimer Export Format PT J TI Response of Listeria monocytogenes to liquid smoke BT AF GUILBAUD, Morgan CHAFSEY, Ingrid PILET, Marie-France LEROI, Francoise PREVOST, Herve HEBRAUD, Michel DOUSSET, Xavier AS 1:1;2:2;3:1;4:3;5:1;6:2;7:1; FF 1:;2:;3:;4:PDG-DOP-DCN-STAM;5:;6:;7:; C1 ENITIAA, LMAI, INRA, UMR 1014,SECALIM, F-44322 Nantes 3, France. INRA Clermont Ferrand Theix, UR454 Microbiol Equipe Qual & Secur Aliments, St Genes Champanelle, France. Ifremer, Dept Sci & Tech Alimentaires Marines, Nantes 03, France. C2 ENITIAA, FRANCE INRA, FRANCE IFREMER, FRANCE SI NANTES SE PDG-DOP-DCN-STAM IN WOS Ifremer jusqu'en 2018 copubli-france copubli-p187 IF 2.028 TC 20 UR https://archimer.ifremer.fr/doc/2008/publication-4522.pdf LA English DT Article DE ;Survival;Stress;Proteome analysis;Listeria monocytogenes;Liquid smoke;Haemolysis AB Aims: To investigate the effect of liquid smoke on growth, survival, proteomic pattern and haemolytic potential of Listeria monocytogenes. Methods and Results: Growth and survival curves were recorded in brain-heart infusion broth supplemented with three concentrations of liquid smoke. L. monocytogenes growth was inhibited in the presence of 15 mu g ml(-1) phenol while a rapid decrease in cell viability occurred in the presence of 30 mu g ml(-1) phenol. The proteome of L. monocytogenes cytosoluble proteins was slightly modified after 2-h incubation with 30 mu g ml(-1) phenol but no protein already characterized in response to other known stresses was induced, except the protease ClpP. Liquid smoke inhibited the haemolytic potential without affecting hly gene expression, showing a potential inhibition of protein activity or stability. Conclusions: The presence of liquid smoke in a rich medium strongly affected growth and survival of L. monocytogenes. Brief smoke stress affected the metabolic pathways and inhibited the haemolytic activity of L. monocytogenes. Significance and Impact of the Study: This study is a first step in the investigation of the influence of a smoked product on L. monocytogenes strains. PY 2008 PD JUL SO Journal of Applied Microbiology SN 1364-5072 PU Blackwell science VL 104 IS 6 UT 000256100700023 BP 1744 EP 1753 DI 10.1111/j.1365-2672.2008.03731.x ID 4522 ER EF