Simultaneous analysis of microbial identity and function using NanoSIMS

Type Article
Date 2008-03
Language English
Author(s) Li T1, 2, Wu T3, 4, Mazeas L1, Toffin LaurentORCID1, 5, Guerquin Kern J3, 4, Leblon G2, Bouchez T1
Affiliation(s) 1 : Cemagref, Unite Rech Hydrosyst & Bioprocedes, F-92163 Antony, France.
2 : Univ Paris 11, Inst Genet & Microbiol, F-91405 Orsay, France.
3 : Inst Curie, Lab Microscopie Ion, F-91405 Orsay, France.
4 : INSERM, U759, F-91405 Orsay, France.
5 : IFREMER, Ctr Brest, Lab Microbiol Environm Extremes UMR6197, F-29280 Plouzane, France.
Source Environmental Microbiology (1462-2912) (Blackwell science), 2008-03 , Vol. 10 , N. 3 , P. 580-588
DOI 10.1111/j.1462-2920.2007.01478.x
WOS© Times Cited 141
Abstract Identifying the function of uncultured microbes in their environments today remains one of the main challenges for microbial ecologists. In this article, we describe a new method allowing simultaneous analysis of microbial identity and function. This method is based on the visualization of oligonucleotide probe-conferred hybridization signal in single microbial cells and isotopic measurement using high-resolution ion microprobe (NanoSIMS). In order to characterize the potential of the method, an oligonucleotide containing iodized cytidine was hybridized on fixed cells of Escherichia coli cultured on media containing different levels of C-13 or N-15. Iodine signals could clearly be localized on targeted cells and the isotopic enrichment could be monitored at the single-cell level. The applicability of this new technique to the study of in situ ecophysiology of uncultured microorganisms within complex microbial communities is illustrated.
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