FN Archimer Export Format
PT J
TI Simultaneous analysis of microbial identity and function using NanoSIMS
BT 
AF LI, T
   WU, T
   MAZEAS, L
   TOFFIN, Laurent
   GUERQUIN KERN, J
   LEBLON, G
   BOUCHEZ, T
AS 1:1,2;2:3,4;3:1;4:1,5;5:3,4;6:2;7:1;
FF 1:;2:;3:;4:PDG-DOP-DCB-EEP-LMEE;5:;6:;7:;
C1 Cemagref, Unite Rech Hydrosyst & Bioprocedes, F-92163 Antony, France.
   Univ Paris 11, Inst Genet & Microbiol, F-91405 Orsay, France.
   Inst Curie, Lab Microscopie Ion, F-91405 Orsay, France.
   INSERM, U759, F-91405 Orsay, France.
   IFREMER, Ctr Brest, Lab Microbiol Environm Extremes UMR6197, F-29280 Plouzane, France.
C2 CEMAGREF, FRANCE
   UNIV PARIS 11, FRANCE
   INST CURIE, FRANCE
   INSERM, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-DOP-DCB-EEP-LMEE
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-univ-france
IF 4.707
TC 151
UR https://archimer.ifremer.fr/doc/2008/publication-4553.pdf
LA English
DT Article
AB Identifying the function of uncultured microbes in their environments today remains one of the main challenges for microbial ecologists. In this article, we describe a new method allowing simultaneous analysis of microbial identity and function. This method is based on the visualization of oligonucleotide probe-conferred hybridization signal in single microbial cells and isotopic measurement using high-resolution ion microprobe (NanoSIMS). In order to characterize the potential of the method, an oligonucleotide containing iodized cytidine was hybridized on fixed cells of Escherichia coli cultured on media containing different levels of C-13 or N-15. Iodine signals could clearly be localized on targeted cells and the isotopic enrichment could be monitored at the single-cell level. The applicability of this new technique to the study of in situ ecophysiology of uncultured microorganisms within complex microbial communities is illustrated.
PY 2008
PD MAR
SO Environmental Microbiology
SN 1462-2912
PU Blackwell science
VL 10
IS 3
UT 000252712300004
BP 580
EP 588
DI 10.1111/j.1462-2920.2007.01478.x
ID 4553
ER

EF