FN Archimer Export Format
PT J
TI New insigths on the metabolic diversity among the epibiotic microbial communitiy of the hydrothermal shrimp Rimicaris exoculata
BT 
AF ZBINDEN, M
   SHILLITO, B
   LE BRIS, Nadine
   DE MONTLAUR, C
   ROUSSEL, Erwan
   GUYOT, F
   GAILL, F
   CAMBON-BONAVITA, Marie-Anne
AS 1:1;2:1;3:2;4:1;5:3;6:4;7:1;8:3;
FF 1:;2:;3:PDG-DOP-DCB-EEP-LEP;4:;5:PDG-DOP-DCB-EEP-LMEE;6:;7:;8:PDG-DOP-DCB-EEP-LMEE;
C1 UPMC, CNRS, UMR 7138, F-75252 Paris 05, France.
   IFREMER, Ctr Brest, Dept Environm Profond, F-29280 Plouzane, France.
   IFREMER, Ctr Brest, Lab Microbiol Extreme Environm, LM2E,UMR 6197, F-29280 Plouzane, France.
   Univ Paris Jussieu, Inst Phys Globe, Lab Mineral Cristallog, F-75252 Paris 05, France.
C2 UNIV PARIS 06, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
   UNIV PARIS 07, FRANCE
SI BREST
SE PDG-DOP-DCB-EEP-LEP
   PDG-DOP-DCB-EEP-LMEE
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.074
TC 66
TU Centre national de la recherche scientifique
   Institut de recherche pour le développement
   Institut français de recherche pour l'exploitation de la mer
   Muséum national d'histoire naturelle
   Université Nice - Sophia-Antipolis
   Université Pierre et Marie Curie
   Université de Bretagne Occidentale
   Université des Antilles
UR https://archimer.ifremer.fr/doc/2008/publication-4563.pdf
LA English
DT Article
CR ATOS
BO Unknown
DE ;Sulfur;Methane;Iron;Intracellular granules;Hydrothermal vent shrimp;High pressure experiments
AB The Rimicaris exoculata dominates the megafauna of some of the Mid Atlantic ridge hydrothermal vent sites. This species harbors a rich community of bacterial epibionts inside its gill chamber. Literature data indicate that a single 16S rRNA phylotype dominates this epibiotic community, and is assumed to be a sulfide-oxidizing bacteria. However attempts Of Cultivation were not successful and did not allow to confirm it. The aim of our study was to test the hypothesis of sulfide oxidation in the gill chamber, by a multidisciplinary approach, using in vivo experiments at in situ pressure in the presence of sulfide, microscopic observations and a molecular survey. Morphology of microorganisms, before and after treatment, was analyzed to test the effect of sulfide depletion and re-exposure. Our observations, as well as molecular data indicate a wider diversity than previously described for this shrimp's epibiotic community. We observed occurrence of bacterial intracellular sulfur- and iron-enriched granules and some methanotrophic-like bacteria cells for the first time. Genes that are characteristic of methane-oxidizing (pmoA) and sulfide-oxidizing (APS) bacteria were identified. These results Suggest that three metabolic types (iron, sulfide and methane oxidation) may co-occur within the epibiont community associated with Rimicaris exoculata. As this shrimp colonizes chemically contrasted environments, the relative abundance of each metabolic type could vary according to the local availability of reduced compounds.
PY 2008
PD MAY
SO Journal of Experimental Marine Biology and Ecology
SN 0022-0981
PU Elsevier
VL 359
IS 2
UT 000256743500007
BP 131
EP 140
DI 10.1016/j.jembe.2008.03.009
ID 4563
ER

EF