Temporal study of estrogenic responses of mussel (Mytilus galloprovinciallis) extracts applied to reporter cell lines
|Author(s)||David A1, Dagnino S1, 2, Pichot Yves3, Munaron Dominique3, Escande A1, 2, Casellas C1, Fenet H1, Gomez E1|
|Affiliation(s)||1 : Fac Pharm Montpellier, UMR 5559, F-34093 Montpellier 5, France.
2 : CRLC, Ctr Rech Cancerol Montpellier, INSERM, F-34298 Montpellier, France.
3 : Stn Sete, IFREMER, F-34200 Sete, France.
|Source||Marine Environmental Research (0141-1136) (Elsevier), 2008-07 , Vol. 66 , N. 1 , P. 105-107|
|WOS© Times Cited||3|
|Keyword(s)||ELISA, Reporter cell lines, Mytilus galloprovinciallis, Estradiol, Biomonitoring|
|Abstract||In vitro bioassays would facilitate monitoring of estrogen-like compounds in mussels (Mytilus galloprovincialis) since they tend to accumulate lipophilic compounds in their fat and muscle tissue. However, estradiol (E2) steroid (already identified in mussels) could induce false positive responses. This study focuses on temporal variability in estrogenic responses of mussel extracts and the possible relation of this response with E2 levels. The reproductive cycle and growth were thus monitored for six months in sexed mussels. E2 levels were similar between genders and these levels varied with reproductive stages. human estrogen receptor (hER) activities were similar at all stages except in February. E2 present in male and female extracts accounted for part of the hER activation observed, but no correlation was found between E2 levels and hER activities. (C) 2008 Elsevier Ltd. All rights reserved.|