FN Archimer Export Format
PT J
TI Phylogenetic and morphological characterisation of the green algae infecting blue mussel Mytilus edulis in the North and South Atlantic oceans
BT 
AF RODRIGUEZ, Francisco
   FEIST, Stephen
   GUILLOU, Laure
   HARKESTAD, Lisbeth
   BATEMAN, Kelly
   RENAULT, Tristan
   MORTENSEN, Stein
AS 1:2,3,4;2:;3:2,3;4:1;5:4;6:5;7:1;
FF 1:;2:;3:;4:;5:;6:PDG-DOP-DCN-AGSAE-LGP;7:;
C1 Inst Marine Res, N-5817 Bergen, Norway.
   CNRS, Stn Biol Roscoff, UMR 7144, F-29682 Roscoff, France.
   Univ Paris 06, F-29682 Roscoff, France.
   Cefas, Weymouth DT4 8UB, Dorset, England.
   IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
C2 INST MAR RES, NORWAY
   CNRS, FRANCE
   UNIV PARIS 06, FRANCE
   CEFAS, UK
   IFREMER, FRANCE
SI LA TREMBLADE
SE PDG-DOP-DCN-AGSAE-LGP
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
IF 1.586
TC 25
UR https://archimer.ifremer.fr/doc/2008/publication-4581.pdf
LA English
DT Article
DE ;Phylogeny;Parasitic alga;Mytilus edulis chilensis;Mytilus edulis;Coccomyxa parasitica;Blue mussels
AB Blue mussels Mytilus edulis with shell deformations and green pustules containing parasitic algae were collected at 3 coastal sites (Burøy, Norway; Bockholm, Denmark; Goose Green, Falkland Islands). A comparative study, including mussel histopathology, algal morphology, ultrastructure and phylogenetic position was performed. Green pustules were mainly located in the posterior portion of the mantle and gonad tissues and the posterior adductor muscle. Electron microscopy confirmed the presence of algal cells with similar morphology to Coccomyxa parasitica. Algae were oval shaped with a single nucleus and chloroplast, 1 or 2 mitochondria and a dense granular cytoplasm with a lipid inclusion body, Golgi apparatus and small vesicles. Partial small subunit (SSU) rRNA phylogeny confirmed the inclusion of parasitic algae into the Coccomyxa clade. However, the sequence identity between almost full SSU rRNA sequences of parasitic algae and others in this clade yielded an unexpected result. Green algae from mussels were distant from C. parasitica Culture Collection of Algae and Protozoa (CCAP) strain 216/18 (94% identity), but very similar (99% identity) to C. glaronensis (a lichen endosymbiont) and green endophytes from the tree Ginkgo biloba. The CCAP strain 216/18 was a sister sequence to Nannochloris algae, far from the Coccomyxa clade. These results suggest a misidentification or outgrowth of the original CCAP strain 216/18 by a different 'Nannochloris-like' trebouxiophycean organism. In contrast, our sequences directly obtained from infested mussels could represent the true C. parasitica responsible for the green pustules in blue mussels.
PY 2008
PD SEP
SO Diseases of aquatic organisms
SN 0177-5103
PU Inter-Research
VL 81
UT 000260244500005
BP 231
EP 240
DI 10.3354/dao01956
ID 4581
ER

EF