FN Archimer Export Format
PT J
TI Influence of subtilisin on the adhesion of a marine bacterium which produces mainly proteins as extracellular polymers
BT 
AF LEROY, Celine
   DELBARRE LADRAT, Christine
   GHILLEBAERT, F
   COMPERE, Chantal
   COMBES, D
AS 1:1;2:1;3:;4:2;5:3;
FF 1:PDG-DOP-DCB-BM-BMM;2:PDG-DOP-DCB-BM-BMM;3:;4:PDG-DOP-DCB-ERT-IC;5:;
C1 IFREMER, Ctr Nantes, Lab Biotechnol & Mol Marines, F-44311 Nantes 03, France.
   IFREMER, Ctr Brest Serv Interfaces & Capteurs, F-29280 Plouzane, France.
   Inst Natl Sci Appl, Lab Biotechnol Bioprocedes, UMR CNRS 5504, UMR INRA 792, F-31077 Toulouse, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   INSA, FRANCE
SI NANTES
   BREST
SE PDG-DOP-DCB-BM-BMM
   PDG-DOP-DCB-ERT-IC
IN WOS Ifremer jusqu'en 2018
   copubli-france
IF 2.028
TC 42
UR https://archimer.ifremer.fr/doc/2008/publication-4624.pdf
LA English
DT Article
DE ;Subtilisin;Pseudoalteromonas;Exopolymer;Capsular protein;Biofilm;Antifouling
AB Aims: The nature of exopolymers involved in the adhesion of a marine biofilm-forming bacterium Pseudoalteromonas sp. D41 was investigated to evaluate and understand the antifouling potential of subtilisin.   Methods and Results: The exopolymers of D41 produced by fermentation were analysed by FTIR and SDS-PAGE showing the presence of polysaccharides, glycoproteins and proteins. A high content of proteins was detected both in soluble and capsular fractions. The microscopic observations of fluorescamine and calcofluor stained adhered D41 indicated mainly the presence of proteins in exopolymers produced during adhesion. Subtilisin, the broad spectrum protease, tested in natural sea water and in polystyrene microplates showed that antifouling activity was higher in the prevention of bacterial adhesion than in the detachment of adhered D41 cells.   Conclusions: Overall, these results demonstrate the involvement of proteins in Pseudoalteromonas sp. D41 adhesion and confirm the high antifouling potential of subtilisin.   Significance and Impact of the Study: This study emphasizes the major role of proteins instead of polysaccharides, thus extending our knowledge regarding the nature of extracellular polymers involved in bacterial adhesion. Furthermore, the high antifouling potential of subtilisin evaluated in the very first stages of fouling, bacterial adhesion, could lead to less toxic compounds than organometallic compounds in antifouling paint.
PY 2008
PD SEP
SO Journal of Applied Microbiology
SN 1364-5072
PU Blackwell science
VL 105
IS 3
UT 000258595600019
BP 791
EP 799
DI 10.1111/j.1365-2672.2008.03837.x
ID 4624
ER

EF