Effects of 16 pure hydrocarbons and two oils on haemocyte and haemolymphatic parameters in the Pacific oyster, Crassostrea gigas (Thunberg)
|Author(s)||Bado Nilles A1, 2, Gagnaire Beatrice3, Thomas-Guyon Helene1, Le Floch S2, Renault Tristan3|
|Affiliation(s)||1 : Univ La Rochelle, CNRS, LIENSs Littoral ENvironm & Soc, UMR 6250, F-17000 La Rochelle, France.
2 : Cedre, Ctr Documentat Rech & Expt Pollut Accid Eaux, F-29218 Brest 2, France.
3 : IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
|Source||Toxicology in Vitro (0887-2333) (Elsevier), 2008-09 , Vol. 22 , N. 6 , P. 1610-1617|
|WOS© Times Cited||41|
|Keyword(s)||Toxicity, PAHs, Pacific oyster, Spectrophotometry, Flow cytometry, Haemocyte activity|
|Abstract||The in vitro effects of polycyclic aromatic hydrocarbons (PAHs) on haemocyte and haemolymphatic parameters of the Pacific oyster, Crassostrea gigas, were tested using field concentrations (10(-7) and 10(-9) mg mL(-1)) observed in the Marennes-Oleron Basin (Charente-Maritime, France) and high concentrations (10(-3) and 10(-5) mg mL(-1)) observed during oil spills. As a first step, the effects of pollutants, after a 24 h contamination period, were monitored on individual and pooled haemolymph samples and similar results were observed for both sample types. In a second step, haemolymphs from 45 oysters were withdrawn and pooled. Eighteen pollutants were tested in vitro after a 4 and 24 h contamination period and 10 of them showed significant modulations of the five haemocyte parameters tested. Seven pollutants (fluorene, pyrene, anthracene, phenanthrene, chrysene, indeno[1,2,3-c,d]pyrene and heavy fuel oil (HFO)) induced a decrease in haemocyte mortality. Fluorene, pyrene and HFO significantly decreased phagocytosis activity. Percentage of non-specific esterase positive cells, phenoloxidase activity and lysosome presence were increased by naphthalene, benzo[b]fluoranthene and dibenz[a,h]anthracene, respectively. Modulation of immune parameters in the Pacific oyster by PAHs suggested that PAH pollution may be related to enhanced susceptibility to diseases. (C) 2008 Elsevier Ltd. All rights reserved.|