Effects of 16 pure hydrocarbons and two oils on haemocyte and haemolymphatic parameters in the Pacific oyster, Crassostrea gigas (Thunberg)

Type Article
Date 2008-09
Language English
Author(s) Bado Nilles A1, 2, Gagnaire Beatrice3, Thomas-Guyon Helene1, Le Floch S2, Renault Tristan3
Affiliation(s) 1 : Univ La Rochelle, CNRS, LIENSs Littoral ENvironm & Soc, UMR 6250, F-17000 La Rochelle, France.
2 : Cedre, Ctr Documentat Rech & Expt Pollut Accid Eaux, F-29218 Brest 2, France.
3 : IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
Source Toxicology in Vitro (0887-2333) (Elsevier), 2008-09 , Vol. 22 , N. 6 , P. 1610-1617
DOI 10.1016/j.tiv.2008.04.011
WOS© Times Cited 41
Keyword(s) Toxicity, PAHs, Pacific oyster, Spectrophotometry, Flow cytometry, Haemocyte activity
Abstract The in vitro effects of polycyclic aromatic hydrocarbons (PAHs) on haemocyte and haemolymphatic parameters of the Pacific oyster, Crassostrea gigas, were tested using field concentrations (10(-7) and 10(-9) mg mL(-1)) observed in the Marennes-Oleron Basin (Charente-Maritime, France) and high concentrations (10(-3) and 10(-5) mg mL(-1)) observed during oil spills. As a first step, the effects of pollutants, after a 24 h contamination period, were monitored on individual and pooled haemolymph samples and similar results were observed for both sample types. In a second step, haemolymphs from 45 oysters were withdrawn and pooled. Eighteen pollutants were tested in vitro after a 4 and 24 h contamination period and 10 of them showed significant modulations of the five haemocyte parameters tested. Seven pollutants (fluorene, pyrene, anthracene, phenanthrene, chrysene, indeno[1,2,3-c,d]pyrene and heavy fuel oil (HFO)) induced a decrease in haemocyte mortality. Fluorene, pyrene and HFO significantly decreased phagocytosis activity. Percentage of non-specific esterase positive cells, phenoloxidase activity and lysosome presence were increased by naphthalene, benzo[b]fluoranthene and dibenz[a,h]anthracene, respectively. Modulation of immune parameters in the Pacific oyster by PAHs suggested that PAH pollution may be related to enhanced susceptibility to diseases. (C) 2008 Elsevier Ltd. All rights reserved.
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Bado Nilles A, Gagnaire Beatrice, Thomas-Guyon Helene, Le Floch S, Renault Tristan (2008). Effects of 16 pure hydrocarbons and two oils on haemocyte and haemolymphatic parameters in the Pacific oyster, Crassostrea gigas (Thunberg). Toxicology in Vitro, 22(6), 1610-1617. Publisher's official version : https://doi.org/10.1016/j.tiv.2008.04.011 , Open Access version : https://archimer.ifremer.fr/doc/00000/4696/