FN Archimer Export Format
PT J
TI Effects of 16 pure hydrocarbons and two oils on haemocyte and haemolymphatic parameters in the Pacific oyster, Crassostrea gigas (Thunberg)
BT 
AF BADO NILLES, A
   GAGNAIRE, Beatrice
   THOMAS-GUYON, Helene
   LE FLOCH, S
   RENAULT, Tristan
AS 1:1,2;2:3;3:1;4:2;5:3;
FF 1:;2:PDG-DOP-DCN-AGSAE-LGP;3:;4:;5:PDG-DOP-DCN-AGSAE-LGP;
C1 Univ La Rochelle, CNRS, LIENSs Littoral ENvironm & Soc, UMR 6250, F-17000 La Rochelle, France.
   Cedre, Ctr Documentat Rech & Expt Pollut Accid Eaux, F-29218 Brest 2, France.
   IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
C2 UNIV LA ROCHELLE, FRANCE
   CEDRE, FRANCE
   IFREMER, FRANCE
SI LA TREMBLADE
   UNIV-FRANCAISE
SE PDG-DOP-DCN-AGSAE-LGP
   PDG-DOP-DCN-AGSAE-CRELA
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.473
TC 43
UR https://archimer.ifremer.fr/doc/2008/publication-4696.pdf
LA English
DT Article
DE ;Toxicity;PAHs;Pacific oyster;Spectrophotometry;Flow cytometry;Haemocyte activity
AB The in vitro effects of polycyclic aromatic hydrocarbons (PAHs) on haemocyte and haemolymphatic parameters of the Pacific oyster, Crassostrea gigas, were tested using field concentrations (10(-7) and 10(-9) mg mL(-1)) observed in the Marennes-Oleron Basin (Charente-Maritime, France) and high concentrations (10(-3) and 10(-5) mg mL(-1)) observed during oil spills. As a first step, the effects of pollutants, after a 24 h contamination period, were monitored on individual and pooled haemolymph samples and similar results were observed for both sample types. In a second step, haemolymphs from 45 oysters were withdrawn and pooled. Eighteen pollutants were tested in vitro after a 4 and 24 h contamination period and 10 of them showed significant modulations of the five haemocyte parameters tested. Seven pollutants (fluorene, pyrene, anthracene, phenanthrene, chrysene, indeno[1,2,3-c,d]pyrene and heavy fuel oil (HFO)) induced a decrease in haemocyte mortality. Fluorene, pyrene and HFO significantly decreased phagocytosis activity. Percentage of non-specific esterase positive cells, phenoloxidase activity and lysosome presence were increased by naphthalene, benzo[b]fluoranthene and dibenz[a,h]anthracene, respectively. Modulation of immune parameters in the Pacific oyster by PAHs suggested that PAH pollution may be related to enhanced susceptibility to diseases. (C) 2008 Elsevier Ltd. All rights reserved.
PY 2008
PD SEP
SO Toxicology in Vitro
SN 0887-2333
PU Elsevier
VL 22
IS 6
UT 000259547300027
BP 1610
EP 1617
DI 10.1016/j.tiv.2008.04.011
ID 4696
ER

EF