FN Archimer Export Format PT J TI Assessment of biochemical methods to detect enzymatic depolymerization of polysaccharides BT AF RIGOUIN, Coraline DELBARRE LADRAT, Christine SINQUIN, Corinne COLLIEC-JOUAULT, Sylvia DION, M AS 1:1,2;2:1;3:1;4:1;5:2; FF 1:PDG-DOP-DCB-BM-BMM;2:PDG-DOP-DCB-BM-BMM;3:PDG-DOP-DCB-BM-BMM;4:PDG-DOP-DCB-BM-BMM;5:; C1 IFREMER, Lab Biotechnol & Mol Marines, F-44311 Nantes 3, France. Univ Nantes, Fac Sci & Tech, CNRS, UMR 6204, F-44322 Nantes, France. C2 IFREMER, FRANCE UNIV NANTES, FRANCE SI NANTES SE PDG-DOP-DCB-BM-BMM IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 3.167 TC 22 UR https://archimer.ifremer.fr/doc/2008/publication-4723.pdf LA English DT Article DE ;detection;enzyme;depolymerization;carbohydrate AB To find biocatalyst allowing depolymerization of new polysaccharides, one needs to get a sensitive and well adapted method to a screening program. This led us to compare biochemical methods of detection of the depolymerization. Currently used methods such as reducing sugars assays, double bond monitoring or molecular weight determination were tested to follow the kinetic of depolymerization with different enzyme / polysaccharide couples. The range of concentrations of assorted enzymes allowed us to identify the most sensitive and appropriate method to detect polysaccharide degradation. Reducing sugars assays are quantitative, sensitive and almost usable with all kind of polysaccharide but some compounds may interfere with them. When polysaccharide is bearing charges, agarose gel electrophoresis, although being a qualitative assay is as sensitive as high performance size exclusion chromatography analysis, easy to handle, quite high-throughput and thus preferred. PY 2009 PD MAR SO Carbohydrate Polymers SN 0144-8617 PU Elsevier VL 76 IS 2 UT 000264229500015 BP 279 EP 284 DI 10.1016/j.carbpol.2008.10.022 ID 4723 ER EF