FN Archimer Export Format
PT J
TI Validation of host-specific Bacteriodales 16S rRNA genes as markers to determine the origin of faecal pollution in Atlantic Rim countries of the European Union
BT 
AF GAWLER, A
   BEECHER, J
   BRANDAO, J
   CARROLL, N
   FALCAO, L
   GOURMELON, Michele
   MASTERSON, B
   NUNES, B
   PORTER, J
   RINCE, A
   RODRIGUES, R
   THORP, M
   WALTERS, J
   MEIJER, W
AS 1:2;2:6;3:4;4:1;5:4;6:3;7:1;8:4;9:2,6;10:3;11:4;12:1;13:2;14:1;
FF 1:;2:;3:;4:;5:;6:PDG-DOP-DCN-EMP-MIC;7:;8:;9:;10:;11:;12:;13:;14:;
C1 Univ Coll Dublin, Sch Geog Planning & Environm Policy, Dublin 4, Ireland.
   Starcross Lab, Natl Lab Serv, Environm Agcy, Starcross Exeter EX6 8PE, Devon, England.
   Microbiol Lab, EMP, Plouzane, France.
   Inst Nacl Saude Dr Ricardo Jorge, P-1649016 Lisbon, Portugal.
   INRA, USC 2017, Lab Microbiol Environm, Caen, France.
   Univ Exeter, Sch Biol & Chem Sci, Exeter EX4 4PS, Devon, England.
C2 UNIV COLL DUBLIN, IRELAND
   ENVIRONM AGENCY, UK
   IFREMER, FRANCE
   INSA LISBON, PORTUGAL
   INRA, FRANCE
   UNIV EXETER, UK
SI BREST
SE PDG-DOP-DCN-EMP-MIC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-europe
IF 3.427
TC 83
UR https://archimer.ifremer.fr/doc/2007/publication-4957.pdf
LA English
DT Article
DE ;Sensitivity;Specificity;PCR;Microbial source tracking;Bacteriodales
AB The recent implementation of the Revised Bathing Water Directive in the European Union has highlighted the need for development of effective methods to differentiate between sources of faecal contamination. It had previously been shown that amplification of 16S rRNA genes of host-specific Bacteriodales species using the HF183F and CF128F primers could be used as markers for human and bovine faecal contamination in the United States. This paper determined the sensitivity and specificity of these markers in four Atlantic Rim countries (France, Ireland, Portugal and the United Kingdom) to evaluate their usefulness in determining the origin of faecal contamination. It was shown that the HF183F marker displayed high sensitivity (80-100%) and specificity (91-100%), and is reliable as an indication of human faecal contamination. The CF128F marker displayed 100% sensitivity in all four countries. However, strong regional variations in specificity (41-96%) were observed, highlighting the need for local validation before this marker is employed in source tracking of faecal contamination. (c) 2007 Elsevier Ltd. All rights reserved.
PY 2007
PD AUG
SO Water Research
SN 0043-1354
PU Elsevier
VL 41
IS 16
UT 000249060100026
BP 3780
EP 3784
DI 10.1016/j.watres.2007.01.028
ID 4957
ER

EF