FN Archimer Export Format
PT J
TI Molecular and phenotypic characterization of Vibrio aestuarianus subsp francensis subsp nov., a pathogen of the oyster Crassostrea gigas
BT 
AF GARNIER, Matthieu
   LABREUCHE, Yannick
   NICOLAS, Jean-Louis
AS 1:1;2:1;3:1;
FF 1:PDG-DOP-DCB-PFOM-PI;2:PDG-DOP-DCOP-LEADNC-LAC;3:PDG-DOP-DCB-PFOM-PI;
C1 IFREMER, Ctr Brest, Lab Physiol Invertebres, PFOM,UMR PE2M, F-29280 Plouzane, France.
C2 IFREMER, FRANCE
SI BREST
   SAINT VINCENT
SE PDG-DOP-DCB-PFOM-PI
   PDG-DOP-DCOP-LEADNC-LAC
IN WOS Ifremer jusqu'en 2018
IF 2.582
TC 54
UR https://archimer.ifremer.fr/doc/2008/publication-4961.pdf
LA English
DT Article
DE ;Polyphasic approach;Summer mortalities;Crassostrea gigas;Pathogen;French isolates;Vibrio aestuarianus
AB Eleven Vibrio isolates invading the hemolymph of live and moribund oysters (Crassostrea gigas) collected in the field and from a hatchery in France, were characterized by a polyphasic approach. Phylogenetic analysis of 16S rRNA, gyrB and toxR genes indicated high homogeneity between these strains and the Vibrio aestuarianus type strain (ATCC35048(T)), and confirmed previous 16S rRNA analysis. In contrast, DNA:DNA hybridization was from 61% to 100%, while phenotypic characters and virulence tests showed a large diversity between the strains. Nevertheless, several common characters allowed the isolates to be distinguished from the reference strain. On the basis of several distinct phenotypic characteristics, it is proposed to establish two subspecies within the V. aestuarianus spp. group, V. aestuarianus subsp. aestuarianus [D. Tison, R. Seidler, Vibrio aestuarianus: a new species from estuarine waters and shellfish, Int. J. Syst. Bacteriol. (1983) 699-702] and V. aestuarianus subsp. francensis for these French isolates. The characters that differentiate the new strains from V. aestuarianus subsp. aestuarianus(T) are virulence (positive for 63% of the isolates) and 12:0 fatty acid content. The colonies were smaller and uncoloured, whereas no growth occurred at 35 degrees C or on TCBS, and the strains did not utilize several substrates, including L-serine, alpha-cyclodextrin, D-mannitol, alpha-glycyl-L-aspartic acid, L-threonine and glucose-1-phosphate. (c) 2008 Elsevier GmbH. All rights reserved.
PY 2008
PD OCT
SO Systematic and Applied Microbiology
SN 0723-2020
PU Elsevier
VL 31
IS 5
UT 000260580900004
BP 358
EP 365
DI 10.1016/j.syapm.2008.06.003
ID 4961
ER

EF