FN Archimer Export Format
PT J
TI ISSR as new markers for genetic characterization and evaluation of relationships among phytoplankton
BT 
AF BORNET, Benjamin
   ANTOINE, Elisabeth
   BARDOUIL, Michele
   MARCAILLOU-LE BAUT, Claire
AS 1:;2:;3:;4:;
FF 1:PDG-DEL-MP-PN;2:PDG-DEL-MP-PN;3:PDG-DEL-MP-PN;4:PDG-DEL-MP-PN;
C1 IFREMER, DEL MP PN, Lab Phycotoxines & Nuisances, F-44311 Nantes 3, France.
C2 IFREMER, FRANCE
SI NANTES
SE PDG-DEL-MP-PN
IN WOS Ifremer jusqu'en 2018
IF 0.762
TC 24
UR https://archimer.ifremer.fr/doc/2004/publication-516.pdf
LA English
DT Article
DE ;Tetraselmis;Skeletonema;Pseudo nitzschia;ISSR;Genetic characterization and diversity;Fingerprints;Alexandrium
AB In order to increase the molecular tools and markers needed for the identification of phytoplankton species, the inter simple sequence repeat ( ISSR) fingerprinting was adapted to micro-algae and its use in genetic analysis was demonstrated. Twelve strains, 6 Alexandrium, 4 Pseudo-nitzschia, 1 Skeletonema and 1 Tetraselmis were analysed for the first time with ISSR amplifications. The patterns were highly polymorphic and very reproducible. The 6 primers gave 223 polymorphic markers that clearly and easily distinguished all 12 strains ( mainly toxic ones) and gave 187 polymorphic markers among the Alexandrium and the Pseudo-nitzschia species. ISSR amplifications also indicated a large occurrence of simple sequence repeat ( SSR) in phytoplankton genomes, especially in Pseudo-nitzschia, and show their usefulness to cluster intra and inter species. ISSR markers were found to be good markers for genetic characterization and diversity study and led to consider them as new tools for the survey of phytoplankton.
PY 2004
PD AUG
SO Journal of Applied Phycology
SN 0921-8971
PU Kluwer
VL 16
IS 4
UT 000225022500004
BP 285
EP 290
DI 10.1023/B:JAPH.0000047780.24787.93
ID 516
ER

EF