Caminicella sporogenes gen. nov., sp nov., a novel thermophilic spore-forming bacterium isolated from an East-Pacific Rise hydrothermal vent

Type Article
Date 2002
Language English
Author(s) Alain Karine, Pignet Patricia, Zbinden M, Quillevere M, Duchiron F, Donval Jean-Pierre, Lesongeur Francoise, Raguenes Gerard, Crassous Philippe, Querellou Joel, Cambon-Bonavita Marie-Anne
Affiliation(s) IFREMER, Ctr Brest, Lab Microbiol & Biotechnol Extremophiles, Dept Valorizat Prod, F-29280 Plouzane, France.
IFREMER, Ctr Brest, Lab Geochim & Met, Dept Geosci Marines, F-29280 Plouzane, France.
IFREMER, Ctr Brest, Lab Biotechnol & Mol Marines, Dept Valorizat Prod, F-29280 Plouzane, France.
Lab Biol Cellulaire & Mol Dev, UMR 7622, F-75252 Paris 05, France.
UFR Sci, URCA, Lab Microbiol Ind, F-51687 Reims 2, France.
IFREMER, Ctr Brest, Lab Environm Profond, Direct Ressources Ocean, F-29280 Plouzane, France.
Source International journal of systematic and evolutionary microbiology (1466-5026) (Society for General Microbiology), 2002 , Vol. 52 , P. 1621-1628
DOI 10.1099/ijs.0.02142-0
WOS© Times Cited 49
Keyword(s) Caminicella sporogenes, Clostridiales, Thermophile, Deep sea hydrothermal vent
Abstract A novel thermophilic, anaerobic, strictly chemoorganoheterotrophic bacterium, designated as AM1114(T), was isolated from a deep-sea hydrothermal vent sample from the East-Pacific Rise (EPR 13degrees N). The cells were long (3-10 mum) rods, motile with peritrichous flagella, and exhibited a Gram-negative cell wall ultrastructure. In the late stationary phase of growth, cells formed an ovoid, refractile, terminal endospore. They grew at 45-65 degreesC inclusive (optimum 55-60 degreesC; doubling time approx. 45 min), at pH 4.5-8.0 inclusive (optimum pH 7.5-8.0) and at sea salt concentrations of 20-60 g l(-1) inclusive (optimum 25-30 g l(-1)). Strain AM1114(T) was an obligately heterotrophic bacterium able to ferment a mixture of 20 amino acids, complex proteinaceous substrates (such as yeast extract, brain-heart infusion or peptone), and carbohydrates such as glucose, galactose or maltose. The main fermentation products on glucose/yeast extract/peptone/sulfur medium were hydrogen, carbon dioxide, butyrate, ethanol, acetate, formate and L-alanine. The G+C content of the genomic DNA (determined by thermal denaturation) was 24.2+/-1 mol %. Phylogenetic analyses of the 16S rRNA gene located the strain within cluster XI of the lineage encompassing the genus Clostridium and related genera (sensu Collins et al., 1994), in the bacterial domain. On the basis of 16S rDNA sequence comparisons and physiological and biochemical characteristics, it is proposed that the isolate should be described as a novel genus, namely Caminicella gen. nov., of which Caminicella sporogenes sp. nov. is the type species. The type strain is AM114(T) (= DSM 14501(T) = CIP 107141(T)).
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Alain Karine, Pignet Patricia, Zbinden M, Quillevere M, Duchiron F, Donval Jean-Pierre, Lesongeur Francoise, Raguenes Gerard, Crassous Philippe, Querellou Joel, Cambon-Bonavita Marie-Anne (2002). Caminicella sporogenes gen. nov., sp nov., a novel thermophilic spore-forming bacterium isolated from an East-Pacific Rise hydrothermal vent. International journal of systematic and evolutionary microbiology, 52, 1621-1628. Publisher's official version : https://doi.org/10.1099/ijs.0.02142-0 , Open Access version : https://archimer.ifremer.fr/doc/00000/528/