Nisaea denitrificans gen. nov., sp nov and Nisaea nitritireducens sp nov., two novel members of the class Alphaproteobacteria from the Mediterranean Sea
|Author(s)||Urios L1, 2, Michotey V3, Intertaglia L1, Lesongeur Francoise4, Lebaron P1|
|Affiliation(s)||1 : Univ Paris 06, Observ Oceanol Banyuls, Lab ARAGO, UMR 7621, F-75005 Paris, France.
2 : INSU CNRS, UMR 7621, F-66650 Banyuls Sur Mer, France.
3 : CNRS, Ctr Oceanol Marseille, Lab Microbiol Geochim & Ecol Marines, UMR 6117, F-13288 Marseille, France.
4 : IFREMER, Ctr Brest, Lab Microbiol Environm Extremes, UMR 6197, F-29280 Plouzane, France.
|Source||International journal of systematic and evolutionary microbiology (1466-5026) (Society for General Microbiology), 2008-10 , Vol. 58 , P. 2336-2341|
|WOS© Times Cited||25|
|Abstract||Two novel Gram-negative bacteria, designated strains DR41_21(T) and DR41_18(T), were isolated from coastal, surface waters of the north-western Mediterranean Sea. The cells were motile, pleomorphic rods, 2.9 mu m long and 0.9 mu m wide and formed cream colonies on marine agar medium. The G + C content of the genomic DNA was 60 mol%. Phylogenetic analysis of 16S rRNA gene sequences positioned the isolates in the class Alphaproteobacteria within the family Rhodospirillaceae. The 16S rRNA gene sequence similarity of the two strains was 98.8% but DNA-DNA hybridization indicated only 55% relatedness. Strain DR41_21(T) was able to denitrify and possessed nirK and nosZ genes, unlike strain DR41_18(T), which possessed only nirK These isolates represent two novel species of a new genus, Nisaea gen. nov., for which the names Nisaea denitrificans sp. nov. and Nisaea nitritireducens sp. nov. are proposed. The type strain of Nisaea denitrificans is DR41_21(T) (=DSM 18348(T) = CIP 109265(T) = OOB 129(T)) and the type strain of Nisaea nitritireducens is DR41_18(T) (=DSM 19540(T) = CIP 10960(T) = OOB 128(T)).|