TY - JOUR T1 - Nisaea denitrificans gen. nov., sp nov and Nisaea nitritireducens sp nov., two novel members of the class Alphaproteobacteria from the Mediterranean Sea A1 - Urios,L A1 - Michotey,V A1 - Intertaglia,L A1 - Lesongeur,Francoise A1 - Lebaron,P AD - Univ Paris 06, Observ Oceanol Banyuls, Lab ARAGO, UMR 7621, F-75005 Paris, France. AD - INSU CNRS, UMR 7621, F-66650 Banyuls Sur Mer, France. AD - CNRS, Ctr Oceanol Marseille, Lab Microbiol Geochim & Ecol Marines, UMR 6117, F-13288 Marseille, France. AD - IFREMER, Ctr Brest, Lab Microbiol Environm Extremes, UMR 6197, F-29280 Plouzane, France. UR - https://archimer.ifremer.fr/doc/00000/5294/ DO - 10.1099/ijs.0.64592-0 N2 - Two novel Gram-negative bacteria, designated strains DR41_21(T) and DR41_18(T), were isolated from coastal, surface waters of the north-western Mediterranean Sea. The cells were motile, pleomorphic rods, 2.9 mu m long and 0.9 mu m wide and formed cream colonies on marine agar medium. The G + C content of the genomic DNA was 60 mol%. Phylogenetic analysis of 16S rRNA gene sequences positioned the isolates in the class Alphaproteobacteria within the family Rhodospirillaceae. The 16S rRNA gene sequence similarity of the two strains was 98.8% but DNA-DNA hybridization indicated only 55% relatedness. Strain DR41_21(T) was able to denitrify and possessed nirK and nosZ genes, unlike strain DR41_18(T), which possessed only nirK These isolates represent two novel species of a new genus, Nisaea gen. nov., for which the names Nisaea denitrificans sp. nov. and Nisaea nitritireducens sp. nov. are proposed. The type strain of Nisaea denitrificans is DR41_21(T) (=DSM 18348(T) = CIP 109265(T) = OOB 129(T)) and the type strain of Nisaea nitritireducens is DR41_18(T) (=DSM 19540(T) = CIP 10960(T) = OOB 128(T)). Y1 - 2008/10 PB - Society for General Microbiology JF - International journal of systematic and evolutionary microbiology SN - 1466-5026 VL - 58 SP - 2336 EP - 2341 ID - 5294 ER -