Type |
Article |
Date |
2004-02 |
Language |
English |
Author(s) |
Giacomazzi Sophie1, Leroi Francoise1, L'Henaff Cecile1, 2, Joffraud Jean-Jacques1 |
Affiliation(s) |
1 : IFREMER, Lab Genie Alimentaire, F-44311 Nantes 3, France. 2 : ENITIAA, Nantes, France. |
Source |
Letters in Applied Microbiology (0266-8254) (Blackwell science), 2004-02 , Vol. 38 , N. 2 , P. 130-134 |
DOI |
10.1111/j.1472-765X.2003.01463.x |
WOS© Times Cited |
12 |
Keyword(s) |
TTGE, RpoB, Nested PCR, Cold smoked salmon flora |
Abstract |
Aims: To evaluate rpoB gene as a biomarker of microbial biodiversity associated to cold-smoked salmon by a novel nested-polymerase chain reaction/temporal temperature gradient gel electrophoresis (PCR/TTGE) technique applied on pure cultures of reference strains. Methods and Results: DNA obtained from pure cultures of reference strains was used in a succession of a first PCR amplification of rpoB fragment with degenerated nonclamped primers and a nested-PCR with nondegenerated clamped primers. PCR products were then applied on a TTGE gel in order to analyse strains profile. High quantity of nested-PCR products were obtained for each tested strain and TTGE profiles showed a good separation between the different reference bacteria and an easy way to associate one band to one species. Conclusion: The nested-PCR/TTGE technique used in this study is a promising way of investigating bacterial community structure of cold-smoked salmon or other food matrix. Significance and Impact of the Study: Because of its single copy state leading to single band profiles in TTGE, rpoB constitute a good potential molecular marker for further development of cold-smoked salmon biodiversity analysis. |
Full Text |
File |
Pages |
Size |
Access |
publication-579.pdf |
13 |
116 KB |
Open access |
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