FN Archimer Export Format
PT J
TI RpoB-PCR amplified gene and temporal temperature gradient gel electrophoresis: a rapid tool to analyse bacterial strains representative of cold-smoked salmon microflora
BT 
AF GIACOMAZZI, Sophie
   LEROI, Francoise
   L'HENAFF, Cecile
   JOFFRAUD, Jean-Jacques
AS 1:1;2:1;3:1,2;4:1;
FF 1:PDG-DRV-VP-GA;2:PDG-DRV-VP-GA;3:PDG-DRV-VP-GA;4:PDG-DRV-VP-GA;
C1 IFREMER, Lab Genie Alimentaire, F-44311 Nantes 3, France.
   ENITIAA, Nantes, France.
C2 IFREMER, FRANCE
   ENITIAA, FRANCE
SI NANTES
SE PDG-DRV-VP-GA
IN WOS Ifremer jusqu'en 2018
   copubli-france
IF 1.461
TC 12
UR https://archimer.ifremer.fr/doc/2004/publication-579.pdf
LA English
DT Article
DE ;TTGE;RpoB;Nested PCR;Cold smoked salmon flora
AB Aims: To evaluate rpoB gene as a biomarker of microbial biodiversity associated to cold-smoked salmon by a novel nested-polymerase chain reaction/temporal temperature gradient gel electrophoresis (PCR/TTGE) technique applied on pure cultures of reference strains.   Methods and Results: DNA obtained from pure cultures of reference strains was used in a succession of a first PCR amplification of rpoB fragment with degenerated nonclamped primers and a nested-PCR with nondegenerated clamped primers. PCR products were then applied on a TTGE gel in order to analyse strains profile. High quantity of nested-PCR products were obtained for each tested strain and TTGE profiles showed a good separation between the different reference bacteria and an easy way to associate one band to one species.   Conclusion: The nested-PCR/TTGE technique used in this study is a promising way of investigating bacterial community structure of cold-smoked salmon or other food matrix.   Significance and Impact of the Study: Because of its single copy state leading to single band profiles in TTGE, rpoB constitute a good potential molecular marker for further development of cold-smoked salmon biodiversity analysis.
PY 2004
PD FEB
SO Letters in Applied Microbiology
SN 0266-8254
PU Blackwell science
VL 38
IS 2
UT 000188411400010
BP 130
EP 134
DI 10.1111/j.1472-765X.2003.01463.x
ID 579
ER

EF