TY - JOUR T1 - Vibrio crassostreae sp nov., isolated from the haemolymph of oysters (Crassostrea gigas) A1 - Faury,Nicole A1 - Saulnier,Denis A1 - Thompson,F A1 - Gay,Melanie A1 - Swings,J A1 - Leroux,Frederique AD - Inst Francais Rech Exploitat Mer, Lab Genet & Pathol, F-17390 La Tremblade, France. AD - State Univ Ghent, Microbiol Lab, B-9000 Ghent, Belgium. AD - State Univ Ghent, BCCM,LMG Bacteria Collect, B-9000 Ghent, Belgium. UR - https://archimer.ifremer.fr/doc/00000/591/ DO - 10.1099/ijs.0.63232-0 KW - Identification KW - Vibrio KW - Crasssotrea gigas KW - Ostreiculture N2 - Polyphasic analysis of five new Vibrio isolates originating from the haernolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus, having 98 % 16S rRNA gene sequence similarity. gyrB phylogenetic analysis, fluorescent amplified-fragment length polymorphism (FAFLP) fingerprinting and DNA-DNA hybridization experiments clearly showed that the new isolates form a tight genomic group that is different from the currently known Vibrio species. It is proposed to accommodate these isolates in a novel species, Vibrio crassostreae sp. nov. (type strain LGP 7(T) =LMG 22240(T)=Clp 108327(T)). Phenotypic and chemotaxonomic features that differentiate V. crassostreae from other known Vibrio species include arginine dihydrolase, utilization and fermentation of various carbon sources, beta-galactosidase activity, NO2 production and the presence of the fatty acids 14: 0 iso and 16: 0 iso. Y1 - 2004/11 PB - Society for General Microbiology JF - International journal of systematic and evolutionary microbiology SN - 1466-5026 VL - 54 IS - 6 SP - 2137 EP - 2140 ID - 591 ER -