Marinilactibacillus piezotolerans sp nov., a novel marine lactic acid bacterium isolated from deep sub-seafloor sediment of the Nankai Trough

Type Article
Date 2005
Language English
Author(s) Toffin Laurent2, 3, Zink Klaus4, Kato Chiaki1, Pignet Patricia5, Bidault Adeline2, 3, Bienvenu Nadège2, 3, Birrien Jean-Louis2, 3, Prieur Daniel2, 3
Affiliation(s) 1 : JAMSTEC, Extremobiosphere Res Ctr, Yokosuka, Kanagawa 2370061, Japan.
2 : Univ Bretagne Occidentale, Technopole Brest Iroise, F-29280 Plouzane, France.
3 : CNRS, UMR 6539, F-29280 Plouzane, France.
4 : Geoforschungszentrum Potsdam, D-14473 Potsdam, Germany.
5 : IFREMER, Ctr Brest, Dept Valorisat Prod, Lab Microbiol & Biotechnol Extremophiles, F-29280 Plouzane, France.
Source International journal of systematic and evolutionary microbiology (1466-5026) (Society for General Microbiology), 2005 , Vol. 55 , N. 1 , P. 345-351
DOI 10.1099/ijs.0.63236-0
WOS© Times Cited 44
Keyword(s) Phylogeny, Biochemistry, Microbiology, Thermophilic bacteria, Deep sub seafloor sediment
Abstract A piezotolerant, mesophilic, marine lactic acid bacterium (strain LT20(T)) was isolated from a deep sub-seafloor sediment core collected at Nankai Trough, off the coast of Japan. Cells were Gram-positive, rod-shaped, non-sporulating and non-motile. The NaGl concentration range for growth was 0-120 gl(-1), with the optimum at 10-20 g l(-1). The temperature range for growth at PH 7.0 was 4-50 degreesC, with the optimum at 37-40 degreesC. The optimum pH for growth was 7.0-8.0. The optimum pressure for growth was 0.1 MPa with tolerance up to 30 MPa. The main cellular phospholipids were phosphatidylglycerols (25 %), diphosphatidylglycerols (34 %) and a group of compounds tentatively identified as ammonium-containing phosphatidylserines (32 %); phosphatidylethanolamines (9 %) were minor components. The fatty acid composition was dominated by side chains of 16:0, 14: 0 and 16: 1. The G +C content of the genomic DNA was 42 mol%. On the basis of 16S rRNA gene sequence analysis and the secondary structure of the V6 region, this organism was found to belong to the genus Marinilactibacillus and was closely related to Marinilactibacillus psychrotolerans M13-2(T) (99 %), Marinilactibacillus sp. strain MJYP.25.24 (99 %) and Alkalibacterium olivapovliticus strain ww2-SN4C (97 %). Despite the high similarity between their 16S rRNA gene sequences (99 %), the DNA-DNA hybridization levels were less than 20 %. On the basis of physiological and genetic characteristics, it is proposed that this organism be classified as a novel species, Marinilactibacillus piezatolerans sp. nov. The type strain is LT20(T) (= DSM 16108(T)=JCM 12337(T)).
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Toffin Laurent, Zink Klaus, Kato Chiaki, Pignet Patricia, Bidault Adeline, Bienvenu Nadège, Birrien Jean-Louis, Prieur Daniel (2005). Marinilactibacillus piezotolerans sp nov., a novel marine lactic acid bacterium isolated from deep sub-seafloor sediment of the Nankai Trough. International journal of systematic and evolutionary microbiology, 55(1), 345-351. Publisher's official version : https://doi.org/10.1099/ijs.0.63236-0 , Open Access version : https://archimer.ifremer.fr/doc/00000/592/