FN Archimer Export Format
PT J
TI Transient initial phase in continuous culture of Isochrysis galbana affinis Tahiti
BT 
AF BOUGARAN, Gael
   LE DEAN, Loic
   LUKOMSKA, Ewa
   KAAS, Raymond
   BARON, Regis
AS 1:;2:;3:;4:;5:;
FF 1:PDG-DRV-VP-PBA;2:PDG-DRV-VP-PBA;3:PDG-DRV-VP-PBA;4:PDG-DRV-VP-PBA;5:PDG-DRV-VP-GA;
C1 IFREMER, F-44311 Nantes 3, France.
C2 IFREMER, FRANCE
SI NANTES
SE PDG-DRV-VP-PBA
   PDG-DRV-VP-GA
IN WOS Ifremer jusqu'en 2018
IF 0.768
TC 16
UR https://archimer.ifremer.fr/doc/2003/publication-597.pdf
LA English
DT Article
DE ;Isochrysis;Cell quota;Photosynthesis;Steady state;Continuous culture
AB Starting microalgae continuous culture generally includes a preliminary batch culture to obtain sufficient cell density. It is possible to apply continuous regime from the beginning of the culture (IC mode) rather than to begin by an initial batch (IB mode). It is our purpose to check that both initial modes lead to identical steady-state cell characteristics. The microalga Isochrysis galbana affinis Tahiti was used for this comparative study. With an initial cell density of 5 x 10(5) cell ml(-1) and a dilution rate of about 1.0 d(-1), both IB and IC modes led to identical cell density once steady-state is reached between 6 and 8 d after inoculation in the two cases. Cell concentration of chlorophyll a and pheopigment a were found to be similar for IB and IC modes at steady-state. Initial culture conditions did not influence saturation irradiance and oxygen consumption rate, which were found to be 650 +/- 143 mumol phot m(-2) s(-1) and 1.54 x 10(-3) +/- 10(-5) mumol O-2 ml(-1) min(-1), respectively. At steady-state. nutrient cell uptakes were p(N) = 83.3 +/- 2.0 fmol N cell(-1) d(-1) for NO3- and rho(p) = 5.5 +/- 0.4 fmol P cell(-1) d(-1) for H2PO4- and did not exhibit significant differences between IB and IC modes. Under the prevailing experimental conditions, results show that IC mode involved very similar steady-state cell characteristics when compared to IIB mode subsequent steady-state. IC mode could be an attractive alternative especially for experimental laboratory studies, as it should lead to higher flexibility in starting continuous cultures. (C) 2003 Editions scientifiques et medicales Elsevier SAS and Ifremer/IRD/Inra/Cemagref. All rights reserved.
PY 2003
PD SEP
SO Aquatic Living Resources
SN 0990-7440
PU Elsevier
VL 16
IS 4
UT 000186027200007
BP 389
EP 394
DI 10.1016/S0990-7440(03)00053-6
ID 597
ER

EF