FN Archimer Export Format
PT J
TI Contribution to toxicity assessment of Dinophysis acuminata (Dinophyceae)
BT 
AF MARCAILLOU-LE BAUT, Claire
   MONDEGUER, Florence
   GENTIEN, Patrick
AS 1:;2:;3:;
FF 1:PDG-DOP-DCN-EMP-PN;2:PDG-DOP-DCN-EMP-PN;3:PDG-DOP-DCN-AGSAE-CREMA;
C1 IFREMER Nantes, F-44311 Nantes, France.
   CREMA lHoumeau, F-17137 Lhoumeau, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
SI NANTES
   LA ROCHELLE
SE PDG-DOP-DCN-EMP-PN
   PDG-DOP-DCN-AGSAE-CREMA
IN WOS Ifremer jusqu'en 2018
IF 0.992
TC 24
UR https://archimer.ifremer.fr/doc/2005/publication-605.pdf
LA English
DT Article
CR DINO IV
   DINOSEINE III
BO Gwen Drez
   Le Suroît
DE ;Liquid chromatography electrospray ionization mass spectrometry;Okadaic acid;Dinophysistoxins;Dinophysis acuminata
AB Blooms of Dinophysis in French coastal waters are implicated in most bans on marketing commercial bivalves. However, the relation between Dinophysis cell density and shellfish toxicity is not always consistent. Discrepancies may be due to the simple fact that it is nearly impossible to compare an integral over a few days (shellfish toxin content) and water samples. Furthermore, it seems that cells may have a variable specific toxicity. This work focuses on the variability in cell toxicity taking into account recent findings and using liquid chromatography coupled to mass spectrometry with an ion trap and electrospray interface. Esterified analogues of okadaic acid (DTX-4 and diol-esters) have been identified in cultures of Prorocentrum lima, another okadaic acid producer. These analogues are inactive on some protein phosphatases, contrary to okadaic acid, and seem to protect the cell from harmful effects by the toxin and to be enzymatically hydrolyzed during cell lysis. In order to document specific toxicity and to validate the presence of these analogues, D. acuminata concentrates were subjected to two separate heating and freeze/thaw procedures, respectively inhibiting or promoting hydrolysis. This paper reports on the high variability of D. acuminata specific toxicity and the presence of esters found in half of the samples only.
PY 2005
PD MAR
SO Journal of Applied Phycology
SN 0921-8971
PU Kluwer
VL 17
IS 2
UT 000230559800008
BP 155
EP 160
DI 10.1007/s10811-005-7907-z
ID 605
ER

EF