TY - JOUR T1 - Cryopreservation of sperm in marine fish A1 - Suquet,Marc A1 - Dreanno,Catherine A1 - Fauvel,Christian A1 - Cosson,J A1 - Billard,R AD - IFREMER, Physiol Poissons Lab, F-29280 Plouzane, France. AD - IFREMER, Stn Expt Aquaculture, F-34250 Palavas Les Flots, France. AD - CNRS, Biol Cellulaire Lab, Stn Marine, F-06230 Villefranche Sur Mer, France. AD - Museum Natl Hist Nat, Lab Ichtyol, F-75231 Paris, France. UR - https://archimer.ifremer.fr/doc/00000/611/ DO - 10.1046/j.1365-2109.2000.00445.x KW - DMSO KW - Insemination KW - Fertilization KW - Cryopreservation KW - Fish sperm N2 - Since the first work of Blaxter in 1953, fish sperm cryopreservation has been attempted on about 30 marine species. The present paper reviews the techniques used and the results published in these species. Particular attention is paid to the handling procedure of sperm before freezing, the problems of semen ageing and semen contamination with urine. The quality of frozen-thawed semen was evaluated using previously standardized biotests, such as a two-step motility activation technique adapted for the different species and fertilization assays using a discriminating insemination technique. Most extenders used in marine fish are saline or sugar solutions. From the investigated cryoprotectants, dimethyl sulphoxide (DMSO) generally leads to the best results. Cooling rates range from 8 degrees C to 99 degrees C min(-1); the thawing rate is generally high. Compared with freshwater species, a high percentage of spermatozoa survives cryopreservation. Therefore, and because of the simplicity of the techniques, the cryopreservation of marine fish sperm is suited for application in aquaculture. Y1 - 2000/03 PB - Blackwell science JF - Aquaculture Research SN - 1355-557X VL - 31 IS - 3 SP - 231 EP - 243 ID - 611 ER -