FN Archimer Export Format PT J TI Cryopreservation of sperm in marine fish BT AF SUQUET, Marc DREANNO, Catherine FAUVEL, Christian COSSON, J BILLARD, R AS 1:;2:;3:;4:;5:; FF 1:PDG-DRV-RA-ARN;2:PDG-DRV-RA-PP;3:PDG-DRV-RA-LPALAVAS;4:;5:; C1 IFREMER, Physiol Poissons Lab, F-29280 Plouzane, France. IFREMER, Stn Expt Aquaculture, F-34250 Palavas Les Flots, France. CNRS, Biol Cellulaire Lab, Stn Marine, F-06230 Villefranche Sur Mer, France. Museum Natl Hist Nat, Lab Ichtyol, F-75231 Paris, France. C2 IFREMER, FRANCE IFREMER, FRANCE CNRS, FRANCE MNHN, FRANCE SI BREST PALAVAS SE PDG-DRV-RA-ARN PDG-DRV-RA-PP PDG-DRV-RA-LPALAVAS IN WOS Ifremer jusqu'en 2018 copubli-france IF 0.469 TC 210 UR https://archimer.ifremer.fr/doc/2000/publication-611.pdf LA English DT Article DE ;DMSO;Insemination;Fertilization;Cryopreservation;Fish sperm AB Since the first work of Blaxter in 1953, fish sperm cryopreservation has been attempted on about 30 marine species. The present paper reviews the techniques used and the results published in these species. Particular attention is paid to the handling procedure of sperm before freezing, the problems of semen ageing and semen contamination with urine. The quality of frozen-thawed semen was evaluated using previously standardized biotests, such as a two-step motility activation technique adapted for the different species and fertilization assays using a discriminating insemination technique. Most extenders used in marine fish are saline or sugar solutions. From the investigated cryoprotectants, dimethyl sulphoxide (DMSO) generally leads to the best results. Cooling rates range from 8 degrees C to 99 degrees C min(-1); the thawing rate is generally high. Compared with freshwater species, a high percentage of spermatozoa survives cryopreservation. Therefore, and because of the simplicity of the techniques, the cryopreservation of marine fish sperm is suited for application in aquaculture. PY 2000 PD MAR SO Aquaculture Research SN 1355-557X PU Blackwell science VL 31 IS 3 UT 000086269000002 BP 231 EP 243 DI 10.1046/j.1365-2109.2000.00445.x ID 611 ER EF