FN Archimer Export Format
PT J
TI Biosynthesis of medium chain length poly-3-hydroxyalkanoates by Pseudomonas guezennei from various carbon sources
BT 
AF SIMON-COLIN, Christelle
   RAGUENES, Gerard
   COSTA, Bernard
   GUEZENNEC, Jean
AS 1:1;2:1;3:2;4:1;
FF 1:PDG-DOP-DCB-BM-BMM;2:PDG-DOP-DCB-BM-BMM;3:;4:PDG-DPCP;
C1 BIOMAR BMM, Inst Francais Rech Exploitat Mer, Ctr Brest, F-29280 Plouzane, France.
   Biolib, F-98701 Tahiti, Polynese Franca, France.
C2 IFREMER, FRANCE
   BIOLIB, FRANCE
SI BREST
SE PDG-DOP-DCB-BM-BMM
   PDG-DPCP
IN WOS Ifremer jusqu'en 2018
   copubli-france
IF 2.039
TC 20
UR https://archimer.ifremer.fr/doc/2008/publication-6115.pdf
LA English
DT Article
DE ;Carbon substrates;Pseudomonas guezennei;Polymer characterization;Polyhydroxyalkanoates
AB The biosynthesis of poly-(3-hydroxyalkanoates) (PHAs) by Pseudomonas guezennei using glucose and/or fatty acids with chain-length from 3 to 18 carbon atoms as carbon sources was investigated. Glucose, acetate, pyruvate, propionate, valerate, hexanoate, heptanoate, octanoate, decanoate, and oleic acid were supplemented in the mineral medium as the sole carbon source or as a mixture for PHAs accumulation. Chemical composition of the resulting PHAs was analysed by coupled gas chromatography mass spectroscopy (GCMS), Fourier Transform Infrared Spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR). PHAs contained up to 10 different types of 3-hydroxyalkanoic acid units, including saturated 3-hydroxyacids from C4 to C14, and unsaturated monomers as 3-hydroxydodecenoate (3HDde) and 3-hydroxytetradecenoate (3HTde). 3-Hydroxydecanaote (3HD) and 3-hydroxyoctanaote (3HO) were incorporated preferentially except during cultivation on heptanoic acid, when A guezennei synthesized PHAs with a higher concentration of 3-hydroxyheptanoate (3HHp). Thermal analysis indicated semi-crystal line polymers with melting temperatures (T-m) ranging from 42 and 51 degrees C, fusion enthalpy (Delta H-m) comprised between 4 and 22 J/g, and glass transition temperatures (T-g) from -41 to -50 degrees C. Crystallinity index (CI) as deduced from FTIR spectra showed values comprised between 0.32 and 0.37. Molecular weights as determined by gel permeation chromatography (GPC-MALLS) ranged from 64200 to 390000 g/mol. The results obtained from this study indicate that P. guezennei is capable of producing a variety of medium chain length (MCL) PHAs via de novo fatty acid biosynthesis and p-oxidation depending on the nature of the carbon source supplied. (c) 2008 Elsevier Ltd. All rights reserved.
PY 2008
PD NOV
SO Reactive and Functional Polymers
SN 1381-5148
PU Elsevier
VL 68
IS 11
UT 000261282800005
BP 1534
EP 1541
DI 10.1016/j.reactfunctpolym.2008.08.005
ID 6115
ER

EF