FN Archimer Export Format PT J TI Effect of the culture system and culture technique on biochemical characteristics of Pavlova lutheri and its nutritional value for Crassostrea gigas larvae BT AF PONIS, Emanuele PARISI, G LE COZ, Jean-Rene ROBERT, Rene ZITTELLI, G TREDICI, R AS 1:1;2:1;3:2;4:3;5:4;6:5; FF 1:PDG-DOP-DCB-PFOM-PI;2:;3:PDG-DOP-DCB-PFOM-PI;4:PDG-DOP-DCB-PFOM-PI;5:;6:; C1 Ifremer, Station Experimentale Argenton, Lab Physiol Invertebres Marins, F-29840 Argenton, France. Univ Florence, Dipartimento Sci Zootecniche, Florence, Italy. Ifremer, Ctr Brest, Lab Physiol Invertebres Marins, F-29280 Plouzane, France. CNR, Ist Studio Ecosistemi, Florence, Italy. Univ Florence, Dipartimento Biotecnol Agrarie, Florence, Italy. C2 IFREMER, FRANCE UNIV FLORENCE, ITALY IFREMER, FRANCE CNR, ITALY UNIV FLORENCE, ITALY SI ARGENTON BREST SE PDG-DOP-DCB-PFOM-PI IN WOS Ifremer jusqu'en 2018 copubli-europe IF 1.642 TC 20 UR https://archimer.ifremer.fr/doc/2006/publication-6117.pdf LA English DT Article DE ;Semi continuous culture;Pavlova lutheri;Flat alveolar photobioreactors;Crassostrea gigas;Carboys;Biochemical composition;Batch culture AB Pavlova lutheri was cultivated in carboys using batch technique and in a flat alveolar photobioreactor (FAP), using initially batch and then semi-continuous techniques. Growth and productivity of the cultures were compared and their nutritional quality was assessed by analysing their biochemical composition (gross composition, fatty acids and sterols) and by evaluating their food value for Pacific oyster (Crassostrea gigas) larvae as bispecific diet in association with Chaetoceros calcitrans forma pumilum. The highest productivity was achieved with the FAP operating in semi-continuous mode. Gross composition of P. lutheri was similar in all cultures, while cellular organic matter content of the microalga grown in FAP in semi-continuous mode was lower. Significant differences in fatty acid content were detected with respect to the n-6 polyunsaturated fatty acid fraction, which was lower in cells cultivated in the FAP in semi-continuous mode. As concerns the main individual fatty acids, docosahexaenoic acid (DHA) content was higher in cells cultivated in the photobioreactor in batch mode than in carboys. The neutral lipid fraction contained seven principal sterols and the comparison among the cultures evidenced different proportions and different patterns. No differences were observed among larvae fed diets containing P. lutheri grown in carboys or in the FAP. PY 2006 PD AUG SO Aquaculture Nutrition SN 1353-5773 PU Wiley / Blackwell VL 12 IS 4 UT 000238741700008 BP 322 EP 329 DI 10.1111/j.1365-2095.2006.00411.x ID 6117 ER EF