A novel mcl-PHA produced on coprah oil by Pseudomonas guezennei biovar. tikehau, isolated from a "kopara" mat of French Polynesia
|Author(s)||Simon-Colin Christelle1, Raguenes Gerard1, Crassous Philippe2, Moppert X3, Guezennec Jean1|
|Affiliation(s)||1 : Ifremer, Ctr Brest, BIOMAR BMM, Lab Biotechnol & Mol Marines, F-29280 Plouzane, France.
2 : Ifremer, Ctr Brest, BIOMAR BMM, Lab Environm Profond, F-29280 Plouzane, France.
3 : Pacific Biotech SARL, F-98701 Tahiti, Polynesie Franc, France.
|Source||International Journal of Biological Macromolecules (0141-8130) (Elsevier), 2008-08 , Vol. 43 , N. 2 , P. 176-181|
|WOS© Times Cited||23|
|Keyword(s)||Polyhydroxyalkanaote, PHAs, Pseudomonas, "kopara", Microbial mat|
|Abstract||Pseudomonasguezennei biovar. tikehau was isolated from a microbial mat on the atoll of Tikehau in French Polynesia, and is able to synthesize medium chain length poly-beta-hydroxyalkanaote copolymers when grown on coprah oil. A two-step cultivation process was used and the biosynthesis of PHAs was followed along 52 h by regular culture sampling. The polyester was purified from freeze-dried cells and analysed by nuclear magnetic resonance (NMR), Fourier transform infra red (FTIR), and gas chromatography mass spectrometries. The copolyester produced by P. guezennei biovar. tikehau from coprah oil mainly consisted of saturated monomers, i.e. 3-hydroxyoctanoate (3HO) and 3-hydroxydecanoate (3HD), and the monomeric composition of the polyester did not change during the fermentation process. However, yield of PHAs production varied from 4% of the cellular dry weight (CDW) to 63% obtained after 36 h. Scan electron microscopy was used to study the morphology and organization of PHAs granules within the cells and revealed the presence of several granules occupying almost the entire cell volume. (C) 2008 Elsevier B.V. All rights reserved.|