FN Archimer Export Format
PT J
TI A novel mcl-PHA produced on coprah oil by Pseudomonas guezennei biovar. tikehau, isolated from a "kopara" mat of French Polynesia
BT 
AF SIMON-COLIN, Christelle
   RAGUENES, Gerard
   CRASSOUS, Philippe
   MOPPERT, X
   GUEZENNEC, Jean
AS 1:1;2:1;3:2;4:3;5:1;
FF 1:PDG-DOP-DCB-BM-BMM;2:PDG-DOP-DCB-BM-BMM;3:PDG-DOP-DCB-EEP-LEP;4:;5:PDG-DOP-DCB-BM-BMM;
C1 Ifremer, Ctr Brest, BIOMAR BMM, Lab Biotechnol & Mol Marines, F-29280 Plouzane, France.
   Ifremer, Ctr Brest, BIOMAR BMM, Lab Environm Profond, F-29280 Plouzane, France.
   Pacific Biotech SARL, F-98701 Tahiti, Polynesie Franc, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   PACIFIC BIOTECH SARL, FRANCE
SI BREST
SE PDG-DOP-DCB-BM-BMM
   PDG-DOP-DCB-EEP-LEP
IN WOS Ifremer jusqu'en 2018
IF 1.867
TC 26
UR https://archimer.ifremer.fr/doc/2008/publication-6119.pdf
LA English
DT Article
DE ;Polyhydroxyalkanaote;PHAs;Pseudomonas;"kopara";Microbial mat
AB Pseudomonasguezennei biovar. tikehau was isolated from a microbial mat on the atoll of Tikehau in French Polynesia, and is able to synthesize medium chain length poly-beta-hydroxyalkanaote copolymers when grown on coprah oil. A two-step cultivation process was used and the biosynthesis of PHAs was followed along 52 h by regular culture sampling. The polyester was purified from freeze-dried cells and analysed by nuclear magnetic resonance (NMR), Fourier transform infra red (FTIR), and gas chromatography mass spectrometries. The copolyester produced by P. guezennei biovar. tikehau from coprah oil mainly consisted of saturated monomers, i.e. 3-hydroxyoctanoate (3HO) and 3-hydroxydecanoate (3HD), and the monomeric composition of the polyester did not change during the fermentation process. However, yield of PHAs production varied from 4% of the cellular dry weight (CDW) to 63% obtained after 36 h. Scan electron microscopy was used to study the morphology and organization of PHAs granules within the cells and revealed the presence of several granules occupying almost the entire cell volume. (C) 2008 Elsevier B.V. All rights reserved.
PY 2008
PD AUG
SO International Journal of Biological Macromolecules
SN 0141-8130
PU Elsevier
VL 43
IS 2
UT 000258480600015
BP 176
EP 181
DI 10.1016/j.ijbiomac.2008.04.011
ID 6119
ER

EF