FN Archimer Export Format
PT J
TI Chromosomal organization of simple sequence repeats in the Pacific oyster (Crassostrea gigas): (GGAT)(4), (GT)(7) and (TA)(10) chromosome patterns
BT 
AF BOUILLY, Karine
   CHAVES, R.
   LEITAO, A.
   BENABDELMOUNA, Abdellah
   GUEDES PINTO, H.
AS 1:1;2:1;3:1,2;4:3;5:1;
FF 1:PDG-DOP-DCN-AGSAE-LGP;2:;3:;4:PDG-DOP-DCN-AGSAE-LGP;5:;
C1 UTAD, CGB, IBB, P-5001801 Vila Real, Portugal.
   So Reg Ctr Fisheries Res CRIP Sul, IPIMAR, P-8700305 Olhao, Portugal.
   IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
C2 UTAD, PORTUGAL
   IPIMAR, PORTUGAL
   IFREMER, FRANCE
SI LA TREMBLADE
SE PDG-DOP-DCN-AGSAE-LGP
IN WOS Ifremer jusqu'en 2018
   copubli-europe
IF 0.64
TC 11
UR https://archimer.ifremer.fr/doc/2008/publication-6132.pdf
LA English
DT Article
DE ;Simple sequence repeats (SSRs);Mollusks;Fluorescence in situ hybridization (FISH);Crassostrea gigas;Chromosome identification
AB Chromosome identification is essential in oyster genomic research. Fluorescence in situ hybridization (FISH) offers new opportunities for the identification of oyster chromosomes. It has been used to locate satellite DNAs, telomeres or ribosomal DNA sequences. However, regarding chromosome identification, no study has been conducted with simple sequence repeats (SSRs). FISH was used to probe the physical organization of three particular SSRs, (GGAT)(4), (GT)(7) and (TA)(10) onto metaphase chromosomes of the Pacific oyster, Crassostrea gigas. Hybridization signals were observed in all the SSR probes, but the distribution and intensity of signals varied according to the oligonucleotide repeat. The intercalary, centromeric and telomeric bands were observed along the chromosomes, and for each particular repeat every chromosome pair presented a similar pattern, allowing karyotypic analysis with all the SSRs tested. Our study is the first in mollusks to show the application of SSR in situ hybridization for chromosome identification and karyotyping. This technique can be a useful tool for oyster comparative studies and to understand genome organization in different oyster taxa.
PY 2008
PD AUG
SO Journal of Genetics
SN 0022-1333
PU Indian Academy of Sciences
VL 87
IS 2
UT 000258990100004
BP 119
EP 125
ID 6132
ER

EF