FN Archimer Export Format
PT J
TI Detection and Quantification of Noroviruses in Shellfish
BT 
AF LE GUYADER, Soizick
   PARNAUDEAU, Sylvain
   SCHAEFFER, Julien
   BOSCH, Albert
   LOISY, Fabienne
   POMMEPUY, Monique
   ATMAR, Robert L.
AS 1:1;2:1;3:1;4:2;5:3;6:1;7:4;
FF 1:PDG-DOP-DCN-EMP-MIC;2:PDG-DOP-DCN-EMP-MIC;3:PDG-DOP-DCN-EMP-MIC;4:;5:;6:PDG-DOP-DCN-EMP;7:;
C1 IFREMER, Microbiol Lab, F-44311 Nantes 03, France.
   Univ Barcelona, Dept Microbiol, Enter Virus Lab, Barcelona, Spain.
   CEERAM SAS, La Chapelle Sur Erdre, France.
   Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA.
C2 IFREMER, FRANCE
   UNIV BARCELONA, SPAIN
   CEERAM SAS, FRANCE
   BAYLOR COLL MED, USA
SI NANTES
   BREST
SE PDG-DOP-DCN-EMP-MIC
   PDG-DOP-DCN-EMP
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-int-hors-europe
IF 3.686
TC 149
UR https://archimer.ifremer.fr/doc/2009/publication-6137.pdf
LA English
DT Article
AB Noroviruses (NoVs) are the most common viral agents of acute gastroenteritis in humans, and high concentrations of NoVs are discharged into the environment. As these viruses are very resistant to inactivation, the sanitary consequences are contamination of food, including molluscan shellfish. There are four major problems with NoV detection in shellfish samples: low levels of virus contamination, the difficulty of efficient virus extraction, the presence of interfering substances that inhibit molecular detection, and NoV genetic variability. The aims of this study were to adapt a kit for use with a method previously shown to be efficient for detection of NoV in shellfish and to use a one step real-time reverse transcription-PCR method with addition of an external viral control. Comparisons of the two methods using bioaccumulated oysters showed that the methods reproducibly detected similar levels of virus in oyster samples. Validation studies using naturally contaminated samples also showed that there was a good correlation between the results of the two methods, and the variability was more attributable to the level of sample contamination. Magnetic silica very efficiently eliminated inhibitors, and use of extraction and amplification controls increased quality assurance. These controls increased the confidence in estimates of NoV concentrations in shellfish samples and strongly supported the conclusion that the results of the method described here reflected the levels of virus contamination in oysters. This approach is important for food safety and is under evaluationfor European regulation.
PY 2009
PD FEB
SO Applied and environmental microbiology
SN 0099-2240
PU American society for microbiology
VL 75
IS 3
UT 000262690100009
BP 618
EP 624
DI 10.1128/AEM.01507-08
ID 6137
ER

EF