FN Archimer Export Format
PT J
TI Effects of commercial enzymes on the adhesion of a marine biofilm-forming bacterium
BT 
AF LEROY, C.
   DELBARRE LADRAT, Christine
   GHILLEBAERT, F.
   COMPERE, Chantal
   COMBES, D.
AS 1:2;2:2;3:3;4:1;5:4;
FF 1:;2:PDG-DOP-DCB-BM-BMM;3:;4:PDG-DOP-DCB-ERT-IC;5:;
C1 IFREMER, Serv Interfaces & Capteurs, Brest, France.
   IFREMER, Lab Biotechnol & Mol Marines, Nantes, France.
   Mexel SA, Verberie, France.
   INSA, CNRS, INRA, Lab Biotechnol Bioprocedes,UMR 5504,UMR 792, Toulouse, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   MEXEL SA, FRANCE
   INSA, FRANCE
SI NANTES
   BREST
SE PDG-DOP-DCB-BM-BMM
   PDG-DOP-DCB-ERT-IC
IN WOS Ifremer jusqu'en 2018
   copubli-france
IF 3.707
TC 112
UR https://archimer.ifremer.fr/doc/2008/publication-6140.pdf
LA English
DT Article
DE ;Marine biofilm;Antifouling;Pseudoalteromonas;Enzymes;Adhesion
AB The antifouling potential of commercial hydrolases, four proteases, seven glycosidases and one lipase was evaluated on the adhesion of marine Pseudoalteromonas sp. D41. The experimental method, adapted to screen antifouling agents, was based on bacterial adhesion in natural sterile sea water in a microtiter plate and on total biomass quantification by the fluorescent dye DAPI (4[prime]6-diamidino-2-phenylindole). Savinase (subtilisin) was the most effective hydrolase in both the prevention of bacterial adhesion and the removal of adhered bacteria. However, some enzymatic preparations tested such as Amano protease were not only ineffective but also increased the number of adhered bacterial cells. Enumeration using epifluorescence microscopy of CTC (5-cyano-2,3-ditolyl tetrazolium chloride) and DAPI stained adhered D41 cells confirmed these observations. Overall, these results demonstrated that hydrolases could either prevent adhesion and remove adhered bacterial cells effectively, or conversely increase bacterial adhesion, depending on enzymatic concentrations and the type of enzymes tested.
PY 2008
SO Biofouling
SN 0892-7014
PU Taylor & Francis
VL 24
IS 1
UT 000252313800002
BP 11
EP 22
DI 10.1080/08927010701784912
ID 6140
ER

EF