FN Archimer Export Format
PT J
TI Archaeal Methane Cycling Communities Associated with Gassy Subsurface Sediments of Marennes-Oleron Bay (France)
BT 
AF ROUSSEL, Erwan
   SAUVADET, Anne Laure
   ALLARD, Jonathan
   CHADUTEAU, Carine
   RICHARD, Pierre
   CAMBON BONAVITA, Marie-Anne
   CHAUMILLON, Eric
AS 1:1;2:1;3:3;4:2;5:3;6:1;7:3;
FF 1:PDG-DOP-DCB-EEP-LMEE;2:;3:;4:PDG-DOP-DCB-GM-LGM;5:;6:PDG-DOP-DCB-EEP-LMEE;7:;
C1 Univ Bretagne Occidentale, Inst Univ Europeen Mer, CNRS, Lab Microbiol Environm Extremes, Plouzane, France.
   Inst Francais Rech Exploitat Mer, Dept Marine Geosci, F-29280 Plouzane, France.
   La Rochelle Univ, CNRS, LIENSS, UMR 6250, La Rochelle, France.
C2 UBO, FRANCE
   IFREMER, FRANCE
   UNIV LA ROCHELLE, FRANCE
SI BREST
SE PDG-DOP-DCB-EEP-LMEE
   PDG-DOP-DCB-GM-LGM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 1.708
TC 30
UR https://archimer.ifremer.fr/doc/2009/publication-6165.pdf
LA English
DT Article
DE ;Sediment;Methane;McrA;16S rRNA;Archaea
AB In Marennes-Oleron Bay, a macro-tidal bay located on the French Atlantic coast, kilometer-scale acoustic turbidity reveals an accumulation of free gas in the sediment. Large concentrations of organic matter and rapid sedimentation rates provide ideal settings for biogenic methane cycling. We integrate seismic, sedimentologic, biogeochemical and molecular genetic approaches to determine whether microbial methane cycling is involved in this process. Here we show that the acoustic turbidity upper boundary matched with X-ray facies displaying fissures with the highest methane concentrations, demonstrating the existence of methane bubbles in the sediment. 16S rRNA and mcrA gene clone libraries were dominated by sequences affiliated to the three known ANME lineages and to putative methanogens. Sequences related to the marine benthic group B (MBG-B) and miscellaneous crenarchaeotal group (MCG) were also detected. However, the highest methane concentration facies was the only section where active Archaea were detected, using reverse-transcribed rRNA, indicating that these communities were involved either directly or indirectly in the methane cycling process. Moreover, three metabolically active novel uncultivated lineages, related to putative methane cycling Archaea, could be specifically associated to these methane bearing sediments. As methane cycling Archaea are commonly retrieved from deep subseafloor and methane seep sediment, the study of coastal gassy sediments, could therefore help to define the biogeochemical habitats of deep biosphere communities.
PY 2009
SO Geomicrobiology Journal
SN 0149-0451
PU Taylor & Francis
VL 26
IS 1
UT 000262296000004
BP 31
EP 43
DI 10.1080/01490450802599284
ID 6165
ER

EF