FN Archimer Export Format PT J TI Cloning of endothelin-1 (ET-1) from European sea bass (Dicentrarchus labrax) and its gene expression analysis in larvae with retinoic acid-induced malformations BT AF GEAY, Florian DARIAS, Maria J. SANTIGOSA, E. DESBRUYERES, Elisabeth QUAZUGUEL, Patrick ZAMBONINO-INFANTE, Jose-Luis CAHU, Chantal MAZURAIS, David AS 1:1;2:1;3:1;4:1;5:1;6:1;7:1;8:1; FF 1:PDG-DOP-DCB-PFOM-ARN;2:;3:;4:PDG-DOP-DCB-PFOM-ARN;5:PDG-DOP-DCB-PFOM-ARN;6:PDG-DOP-DCB-PFOM-ARN;7:PDG-DOP-DCB-PFOM;8:PDG-DOP-DCB-PFOM-ARN; C1 IFREMER, Ifremer Marine Fish Nutr Team, Nutr Aquaculture & Genom Res Unit, UMR 1067, F-29280 Plouzane, France. C2 IFREMER, FRANCE SI BREST SE PDG-DOP-DCB-PFOM-ARN PDG-DOP-DCB-PFOM IN WOS Ifremer jusqu'en 2018 IF 1.925 TC 5 UR https://archimer.ifremer.fr/doc/2009/publication-6172.pdf LA English DT Article DE ;European sea bass;Retinoic acid;Morphogenesis;Gene expression;Endothelin 1 AB It is known in vertebrates that endothelin-1 (ET-1) plays a key role ill morphogenesis whose expression level greatly influences the development of craniofacial malformations. In the Present study, the complete cDNA fragment encoding a precursor of endothelin-1, the preproendothelin-1 (PPET1), was cloned by RACE-PCR from European sea bass. The cDNA encoded a 199 amino acid polypeptide that was composed of the "mature" and "big" ET-1. Relative ET-1 expression levels were investigated in European sea bass larvae fed microparticulate diets containing the standard amount (0.08 g retinol/kg diet: group N) or all excess of retinoic acid which induces skeletal malformations (0.5 g retinol/kg diet: group RA). Real-time reverse transcriptor polymerase chain reaction analysis revealed that PPET1 mRNA levels were sensitively reduced in the RA group during early development (days 10 and 15 post hatching). Regulation of ET-1 gene expression in larvae fed the teratogenic level of vitamin A confirmed the involvement of ET-1 in the molecular mechanism involved in craniofacial deformities. These results suggest that the expression level of ET-1 may be used as a precocious molecular marker to predict malformations during European sea bass development. (c) 2008 Elsevier B.V. All rights reserved. PY 2009 PD FEB SO Aquaculture SN 0044-8486 PU Elsevier VL 287 IS 1-2 UT 000263024200024 BP 169 EP 173 DI 10.1016/j.aquaculture.2008.10.011 ID 6172 ER EF