FN Archimer Export Format PT J TI Long-term ammonia exposure of turbot: effects on plasma parameters BT AF PERSON, Jeannine LAMERS, A LE ROUX, Annick SEVERE, Armelle BOEUF, Gilles MAYER GOSTAN, N AS 1:;2:;3:;4:;5:;6:; FF 1:PDG-DRV-RA-ARN;2:;3:PDG-DRV-RA-PI;4:PDG-DRV-RA-ARN;5:;6:; C1 Ctr Brest, IFREMER, Lab Adaptat Reprod & Nutr, F-29280 Plouzane, France. Univ Nice, UMR INRA UNSA Rose 1112, Fac Sci, F-06108 Nice 2, France. C2 CTR BREST, FRANCE UNIV NICE, FRANCE SI BREST SE PDG-DRV-RA-ARN PDG-DRV-RA-PI IN WOS Ifremer jusqu'en 2018 copubli-univ-france IF 1.2 TC 45 UR https://archimer.ifremer.fr/doc/2003/publication-618.pdf LA English DT Article DE ;Urea;Total ammonia nitrogen;Scophthalmus maximus;Ion regulation;Cortisol;Chronic toxicity AB Turbot juveniles were exposed to four ammonia concentrations [0.17 (L), 0.34 (M), 0.73 (MH) and 0.88 (H) mgl(-1) NH3-N] for different exposure durations (28 days minimum to 84 days). Their physiological status and growth performances were compared to a control group [0.004 (C) mgl(-1) NH3-N]. No growth was observed in the H group, and by day 57, mass increase in the MH group was only 15% of that in group C. During the first month growth in the L group was similar to that in control group while it was lower (33%) in the M group; afterwards the L and M groups had a similar growth (half that of controls). Accumulation of total ammonia nitrogen (TA-N) in plasma was dependent on ambient ammonia concentrations. Plasma urea levels in ammonia-exposed fish were lower, similar or greater than ill controls (depending oil ammonia concentration or exposure duration). Osmolarity, Cl- and Na+ plasma concentrations were stable in the L and M groups. The increases in Na+, Cl-, K+ and total Ca concentrations observed by the end of the experiment in the H and MH groups suggest that fish failed to adapt. There was all initial rise in plasma cortisol in all ammonia-exposed groups followed by a return to basal level (1.7-4 ngml(-1)) in the L and M groups. In group M H, plasma cortisol peaked at 42 ngml(-1) by day 14, and after a decline at c. 1 month (14 ngml(-1)), it rose again. PY 2003 PD APR SO Journal of Fish Biology SN 0022-1112 PU Blackwell science VL 62 IS 4 UT 000185632100011 BP 879 EP 894 DI 10.1046/j.1095-8649.2003.00073.x ID 618 ER EF