FN Archimer Export Format PT J TI Setting of a procedure for experimental fertilisation of Pacific oyster (Crassostrea gigas) oocytes BT AF SONG, Yun-Peng SUQUET, Marc QUEAU, Isabelle LEBRUN, Luc AS 1:1;2:1;3:1;4:1; FF 1:PDG-DOP-DCB-PFOM-PI;2:PDG-DOP-DCB-PFOM-ARN;3:PDG-DOP-DCB-PFOM-PI;4:PDG-DOP-DCB-PFOM-PI; C1 IFREMER, Stn Expt Argenton, Dept PFOM, F-29840 Argenton, France. C2 IFREMER, FRANCE SI ARGENTON SE PDG-DOP-DCB-PFOM-PI PDG-DOP-DCB-PFOM-ARN IN WOS Ifremer jusqu'en 2018 IF 1.925 TC 35 UR https://archimer.ifremer.fr/doc/2009/publication-6231.pdf LA English DT Article DE ;Crassostrea gigas;Pacific oyster;Experimental;Fertilisation AB The quality of oyster spermatozoa has become an issue for modern aquaculture. However, reliable protocols used to assess gamete quality are lacking. The aim of this work is to define a standardised experimental fertilisation protocol for Pacific oyster oocytes. Six experiments have been carried out in this study. The optimal conditions for Pacific oyster experimental fertilisation have been defined: (1) oocytes can be conserved in seawater for at least 4 h before fertilisation. (2) oocyte concentration: 100-1000 ml(-1) seawater, (3) fertilisation volume: 10-100 nil, (4) spermatozoa: oocyte ratio: 400 to maintain discriminating conditions, (5) gamete contact time: longer than 10 mill and (6) fertilisation and incubation temperatures: both 19 degrees C. The effect of individual male significantly influenced the fertilisation success. The results of this study will be exploited for spermatozoal quality analysis in combination with other techniques. (C) 2008 Elsevier B.V. All rights reserved. PY 2009 PD FEB SO Aquaculture SN 0044-8486 PU Elsevier VL 287 IS 3-4 UT 000263388500012 BP 311 EP 314 DI 10.1016/j.aquaculture.2008.10.018 ID 6231 ER EF